Cloned (Comment) | Organism |
---|---|
overexpression of wild-type MTMR2 in HeLa cells predominantly located to the cytosolic/perinuclear region, recombinant expression of FLAG-tagged and of His-tagged wild-type and mutant enzymes in Escherichia coli lysates | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C417S | site-directed mutagenesis, a catalytically inactive MTMR2 variant | Homo sapiens |
S58A | site-directed mutagenesis, a phosphorylation-mimetic mutant, which retains full catalytic activity toward PI(3)P and PI(3,5)P2 as compared to wild-type MTMR2. The majority of the MTMR2 phosphorylation-deficient S58A mutant is localized to distinct punctate structures, indicative of endosomal localization, in contrast to the wild-type enzyme. The level of ERK1/2 phosphorylation/activation is also2-3fold higher in cells expressing MTMR2S58A in comparison to control cells | Homo sapiens |
S58A/C417S | site-directed mutagenesis, a catalytically inactive MTMR2 variant, which shows strong co-localization with markers for early endosomes including the PI(3)P marker generated from EEA1 and Rab5 | Homo sapiens |
S58E | site-directed mutagenesis, a phosphorylation-deficient mutant, which retains full catalytic activity toward PI(3)P and PI(3,5)P2 as compared to wild-type MTMR2. The mutant enzyme displays a similar localization pattern as wild-type in the cytoplasm | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | Homo sapiens | - |
1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | Homo sapiens | - |
1-phosphatidyl-1D-myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene mtmr2 | - |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged and His-tagged wild-type and mutant enzymes from Escherichia coli lysates | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? | |
1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTMR2 | - |
Homo sapiens |
phosphoinositide 3-phosphatase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |