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Literature summary for 3.1.3.64 extracted from
- Production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells (2004), J. Biol. Chem., 279, 7304-7312.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged enzyme in Escherichia coli strain DH5alpha, overexpression in COS-7 cells, HEK-293 cells, L6 cells, C2C12 cells, and Jurkat with 10-15% expression level in all cases | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D278A | site-directed mutagenesis, inactive, substrate-trapping mutant | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | Homo sapiens | - |
1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? |  |
| 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | Homo sapiens | - |
1-phosphatidyl-1D-myo-inositol + phosphate | - |
? | |
| additional information | Homo sapiens | enzyme deficiency is involved in XLMTM myopathies | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q13496 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | - |
Homo sapiens | - |
| myoblast | - |
Homo sapiens | - |
| myotube | fibroblast cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hexanoyl-2-lysophosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | - |
Homo sapiens | 1-hexanoyl-2-lysophosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate | - |
? | |
| 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | - |
Homo sapiens | 1-phosphatidyl-1D-myo-inositol + phosphate | - |
? | |
| hexanoylglyceryl 1-phosphatidyl-1D-myo-inositol 3-phosphate + H2O | - |
Homo sapiens | hexanoylglyceryl 1-phosphatidyl-1D-myo-inositol + phosphate | - |
? | |
| additional information | enzyme deficiency is involved in XLMTM myopathies | Homo sapiens | ? | - |
? | |
| additional information | the enzyme shows specificity for 1-phosphatidyl-1D-myo-inositol 3-phosphate and 1-phosphatidyl-1D-myo-inositol 3,3-bisphosphate independent of the side acyl chain length | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MTM1 | - |
Homo sapiens |
| myotubularin | - |
Homo sapiens |
| phosphoinositide 3-phosphatase | - |
Homo sapiens |
| PtdIns(3)P phosphatase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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