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Literature summary for 3.1.3.64 extracted from
- Isolation and characterization of two 3-phosphatases that hydrolyze both phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate (1991), J. Biol. Chem., 266, 18378-18386.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | maximally active in the presence of EDTA | Rattus norvegicus |  |
| octyl glucoside | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
Inositol 1,3-bisphosphate | type I enzyme | Rattus norvegicus | |
| 0.0037 | - |
Inositol 1,3-bisphosphate | type II enzyme | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
| soluble | predominantly | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, type I 3-phosphatase, SDS-PAGE | Rattus norvegicus |
| 65000 | - |
1 * 65000 + 1 * 78000, type II 3-phosphatase, SDS-PAGE | Rattus norvegicus |
| 78000 | - |
1 * 65000 + 1 * 78000, type II 3-phosphatase, SDS-PAGE | Rattus norvegicus |
| 110000 | - |
type I 3-phosphatase, gel filtration | Rattus norvegicus |
| 147000 | - |
type II 3-phosphatase, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inositol 1,3-bisphosphate + H2O | Rattus norvegicus | type I enzyme may regulate inositol 1,3-bisphosphate levels in cells and is designated inositol-polyphosphate 3-phosphatase | inositol 1-phosphate + phosphate | - |
? | |
| phosphatidylinositol 3-phosphate + H2O | Rattus norvegicus | type II enzyme functions primarily to metabolize phosphatidylinositol 3-phosphate and is designated phosphatidylinositol-3-phosphatase | phosphatidylinositol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
two enzyme forms: type I and type II 3-phosphatase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, two enzyme forms: type I and type II 3-phosphatase | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C, frozen in liquid N2, at least 1 month, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inositol 1,3-bisphosphate + H2O | two enzyme forms, type I and type II 3-phosphatase, with differing relative specificities for phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate, the catalytic efficiency of type I enzyme is 19fold higher than that of type II enzyme | Rattus norvegicus | inositol 1-phosphate + phosphate | - |
? | |
| inositol 1,3-bisphosphate + H2O | type I enzyme may regulate inositol 1,3-bisphosphate levels in cells and is designated inositol-polyphosphate 3-phosphatase | Rattus norvegicus | inositol 1-phosphate + phosphate | - |
? | |
| additional information | not: inositol 1,3,4,5-tetrakisphosphate, inositol 1,3,4-trisphosphate or inositol 3,4-bisphosphate | Rattus norvegicus | ? | - |
? | |
| phosphatidylinositol 3-phosphate + H2O | two enzyme forms, type I and type II 3-phosphatase, with differing relative specificities for phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate, hydrolysis by type II enzyme at 3times the rate of type I enzyme | Rattus norvegicus | phosphatidylinositol + phosphate | - |
? | |
| phosphatidylinositol 3-phosphate + H2O | type II enzyme functions primarily to metabolize phosphatidylinositol 3-phosphate and is designated phosphatidylinositol-3-phosphatase | Rattus norvegicus | phosphatidylinositol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 65000 + 1 * 78000, type II 3-phosphatase, SDS-PAGE | Rattus norvegicus |
| heterodimer | the 65 kDa subunit of the type I and II enzyme appear to be identical, based on co-migration in SDS-polyacrylamide gels and peptide mapping, the 78 kDa subunit of the type II enzyme may be a regulatory subunit | Rattus norvegicus |
| homodimer | 2 * 65000, type I 3-phosphatase, SDS-PAGE | Rattus norvegicus |
| homodimer | the 65 kDa subunit of the type I and II enzyme appear to be identical, based on co-migration in SDS-polyacrylamide gels and peptide mapping | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Rattus norvegicus |
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