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Literature summary for 3.1.3.63 extracted from
- Purification and properties of 2-carboxy-D-arabinitol 1-phosphatase (1989), Plant Physiol., 90, 679-685.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | activation | Nicotiana tabacum |  |
| D-fructose 1,6-bisphosphate | activation | Nicotiana tabacum | |
| additional information | no activation by ATP | Nicotiana tabacum | |
| additional information | no activation by ADP, 6-phosphogluconate, D-fructose 6-phosphate, D-glucose 1-phosphate, D-ribulose 5-phosphate, D-ribose 5-phosphate | Nicotiana tabacum | |
| Ribulose 1,5-bisphosphate | activation | Nicotiana tabacum | |
General Stability
| General Stability | Organism |
|---|---|
| major loss of activity during ion-exchange chromatography | Nicotiana tabacum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Nicotiana tabacum | |
| Cu2+ | 5 mM | Nicotiana tabacum | |
| MoO42- | strong | Nicotiana tabacum | |
| additional information | no inhibition by K+, Mg2+, Na+, NH4+, EDTA or cysteine | Nicotiana tabacum | |
| PO43- | - |
Nicotiana tabacum | |
| ribose 5-phosphate | weak | Nicotiana tabacum | |
| SO42- | inhibitory above 700 mM, activates at 10-50 mM | Nicotiana tabacum | |
| Zn2+ | 5 mM | Nicotiana tabacum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
2-carboxyarabinitol 1-phosphate | 25°C, pH 7.5 | Nicotiana tabacum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Nicotiana tabacum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by Mg2+ | Nicotiana tabacum | |
| additional information | no activation by K+, Na+, NH4+ | Nicotiana tabacum | |
| SO42- | activation, 10-50 mM, inhibits above 700 mM | Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-carboxy-D-arabinitol 1-phosphate + H2O | Nicotiana tabacum | - |
2-carboxy-D-arabinitol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
tobacco, var. KY14 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from leaf and from isolated chloroplasts | Nicotiana tabacum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2612 | - |
25°C, pH 7.5 | Nicotiana tabacum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, slow loss of activity in the absence of ammonium sulfate | Nicotiana tabacum |
| 4°C, stable as ammonium sulfate suspension, slow loss of activity in the absence of ammonium sulfate | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-carboxy-D-arabinitol 1-phosphate + H2O | - |
Nicotiana tabacum | 2-carboxy-D-arabinitol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Nicotiana tabacum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.4 | about 65% of maximal activity at pH 5.5 and about half-maximal activity at pH 8.4 | Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | activation | Nicotiana tabacum | |
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