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Literature summary for 3.1.3.62 extracted from
- Lily pollen alkaline phytase is a histidine phosphatase similar to mammalian multiple inositol polyphosphate phosphatase (MINPP) (2006), Phytochemistry, 67, 1874-1886.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli | Lilium longiflorum |
| expressed in Escherichia coli | Lilium longiflorum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | L1ALP contains the histidine phosphatase conserved sequence, -RHGXRXP-, and therefore belongs to the histidine phosphatase family of enzymes unlike alkaline phytases from bacteria | Lilium longiflorum |
| additional information | two isoforms of alkaline phytase cDNAs, L1Alp1 and L1Alp2, which are 1467 and 1533 bp long and encode proteins of 487 and 511 amino acids, respectively, are identified. Phylogenetic analysis reveals that LlALP is most closely related to multiple inositol polyphosphate phosphatase (MINPP) from humans (25%) and rats (23%) | Lilium longiflorum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Lilium longiflorum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53800 | - |
SDS-PAGE | Lilium longiflorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lilium longiflorum | Q0GYS1 | - |
- |
| Lilium longiflorum | Q0GYS2 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | mRNA expression | Lilium longiflorum | - |
| petal | mRNA expression | Lilium longiflorum | - |
| pollen | mRNA expression | Lilium longiflorum | - |
| stem | mRNA expression | Lilium longiflorum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
L1Alp2 is shown to possess phytase activity | Lilium longiflorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inositol hexakisphosphate + H2O | - |
Lilium longiflorum | inositol pentakisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkaline phytase isoform 1 | - |
Lilium longiflorum |
| alkaline phytase isoform 2 | - |
Lilium longiflorum |
| L1Alp1 | - |
Lilium longiflorum |
| L1Alp2 | - |
Lilium longiflorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lilium longiflorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Lilium longiflorum |
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Release 2023.1 (January 2023)
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