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Literature summary for 3.1.3.6 extracted from

  • Gbenle, G.O.; Dwyer, D.M.
    Purification and properties of 3'-nucleotidase of Leishmania donovani (1992), Biochem. J., 285, 41-46.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
dilution causes loss of activity Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosine 3'-phosphate
-
Leishmania donovani
5'-AMP
-
Leishmania donovani
ADP
-
Leishmania donovani
ATP
-
Leishmania donovani
dithiothreitol
-
Leishmania donovani
EDTA
-
Leishmania donovani
Zn2+
-
Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
3'-AMP
-
Leishmania donovani
0.28
-
3'-IMP
-
Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Leishmania donovani 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-AMP Leishmania donovani processing of exogenously available 3'-nucleotides into a form suitable for transport across the surface membrane of the parasite, which is incapable of purine biosynthesis de novo ?
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2345
-
-
Leishmania donovani

Storage Stability

Storage Stability Organism
0°C, purified enzyme stable Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP processing of exogenously available 3'-nucleotides into a form suitable for transport across the surface membrane of the parasite, which is incapable of purine biosynthesis de novo Leishmania donovani ?
-
?
3'-AMP + H2O 74% of the activity with 3'-IMP Leishmania donovani adenosine + phosphate
-
?
3'-CMP + H2O 6% of the activity with 3'-IMP Leishmania donovani cytosine + phosphate
-
?
3'-GMP + H2O 21% of the activity with 3'-IMP Leishmania donovani guanosine + phosphate
-
?
3'-IMP + H2O best substrate Leishmania donovani inosine + phosphate
-
?
3'-UMP + H2O 37% of the activity with 3'-IMP Leishmania donovani uridine + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5
-
Leishmania donovani