BRENDA - Enzyme Database
show all sequences of 3.1.3.59

1-O-alkyl-2-acetyl-sn-glycero-3-phosphate:phosphohydrolase activity in Tetrahymena pyriformis

Tellis, C.; Lekka, M.E.; J. Eukaryot. Microbiol. 47, 122-128 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Tween 20
20% increase of activity
Tetrahymena pyriformis
Tween 80
20% increase of activity
Tetrahymena pyriformis
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-chloro-2,3-dinitrobenzene
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
acyl-glycerolphosphate
38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
alkyl-glycerolphosphate
58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
Ca2+
-
Tetrahymena pyriformis
dithiothreitol
-
Tetrahymena pyriformis
Mg2+
-
Tetrahymena pyriformis
additional information
no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
Tetrahymena pyriformis
N-ethylmaleimide
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
Nonidet
100% inhibition
Tetrahymena pyriformis
SDS
100% inhibition
Tetrahymena pyriformis
sodium deoxycholate
100% inhibition
Tetrahymena pyriformis
Triton-X100
0.2% causes 100% inhibition
Tetrahymena pyriformis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
pH 7.0, 37C, mitochondrial and microsomal fractions
Tetrahymena pyriformis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
15% of the total activity
Tetrahymena pyriformis
5829
-
membrane
-
Tetrahymena pyriformis
16020
-
microsome
20% of the total activity
Tetrahymena pyriformis
-
-
mitochondrion
40% of the total activity
Tetrahymena pyriformis
5739
-
pellicle
20% of the total activity
Tetrahymena pyriformis
20039
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Tetrahymena pyriformis
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
?
-
-
-
Organic Solvent Stability
Organic Solvent
Commentary
Organism
additional information
EDTA and EGTA no affect on enzyme activity
Tetrahymena pyriformis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tetrahymena pyriformis
-
protozoan
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Tetrahymena pyriformis
0.0031
-
cytosol
Tetrahymena pyriformis
0.0159
-
pellicle
Tetrahymena pyriformis
0.0164
-
mitochondrion and microsome
Tetrahymena pyriformis
Storage Stability
Storage Stability
Organism
-80C, stable for 3 months
Tetrahymena pyriformis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
-
646420
Tetrahymena pyriformis
1-acyl-2-acetyl-sn-glycerol + phosphate
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
best substrate
646420
Tetrahymena pyriformis
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
646420
Tetrahymena pyriformis
?
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
-
646420
Tetrahymena pyriformis
?
-
-
-
?
alkyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
alkylglycerol + phosphate
-
-
-
?
diacyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
dialkylglycerol + phosphate
poor substrate
646420
Tetrahymena pyriformis
?
additional information
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
646420
Tetrahymena pyriformis
?
-
-
-
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
45
stable
Tetrahymena pyriformis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
microsome and mitochondrion
Tetrahymena pyriformis
9
-
microsome
Tetrahymena pyriformis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Tween 20
20% increase of activity
Tetrahymena pyriformis
Tween 80
20% increase of activity
Tetrahymena pyriformis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-chloro-2,3-dinitrobenzene
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
acyl-glycerolphosphate
38% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
alkyl-glycerolphosphate
58% inhibition in presence of 0.2 mM 1-alkyl-2-acetyl-sn-glycero-3-phosphate, mitochondrial fraction
Tetrahymena pyriformis
Ca2+
-
Tetrahymena pyriformis
dithiothreitol
-
Tetrahymena pyriformis
Mg2+
-
Tetrahymena pyriformis
additional information
no inhibition: diacyl-glycerolphosphate, D-fructose-6-phosphate, 2-glycerophosphate, ATP, 1-alkyl-2-acetyl-sn-glycerol
Tetrahymena pyriformis
N-ethylmaleimide
pre-treatment of the membrane fraction with 5 mM for 15 min causes 30% inhibition
Tetrahymena pyriformis
Nonidet
100% inhibition
Tetrahymena pyriformis
SDS
100% inhibition
Tetrahymena pyriformis
sodium deoxycholate
100% inhibition
Tetrahymena pyriformis
Triton-X100
0.2% causes 100% inhibition
Tetrahymena pyriformis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
1-Alkyl-2-acetyl-sn-glycero-3-phosphate
pH 7.0, 37C, mitochondrial and microsomal fractions
Tetrahymena pyriformis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
15% of the total activity
Tetrahymena pyriformis
5829
-
membrane
-
Tetrahymena pyriformis
16020
-
microsome
20% of the total activity
Tetrahymena pyriformis
-
-
mitochondrion
40% of the total activity
Tetrahymena pyriformis
5739
-
pellicle
20% of the total activity
Tetrahymena pyriformis
20039
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Tetrahymena pyriformis
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
?
-
-
-
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
additional information
EDTA and EGTA no affect on enzyme activity
Tetrahymena pyriformis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Tetrahymena pyriformis
0.0031
-
cytosol
Tetrahymena pyriformis
0.0159
-
pellicle
Tetrahymena pyriformis
0.0164
-
mitochondrion and microsome
Tetrahymena pyriformis
Storage Stability (protein specific)
Storage Stability
Organism
-80C, stable for 3 months
Tetrahymena pyriformis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-acyl-2-acetyl-sn-glycero-3-phosphate + H2O
-
646420
Tetrahymena pyriformis
1-acyl-2-acetyl-sn-glycerol + phosphate
-
-
-
?
1-alkyl-2-acetyl-sn-glycero-3-phosphate + H2O
best substrate
646420
Tetrahymena pyriformis
1-alkyl-2-acetyl-sn-glycerol + phosphate
-
646420
Tetrahymena pyriformis
?
1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O
-
646420
Tetrahymena pyriformis
?
-
-
-
?
alkyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
alkylglycerol + phosphate
-
-
-
?
diacyl-glycerophosphate + H2O
-
646420
Tetrahymena pyriformis
dialkylglycerol + phosphate
poor substrate
646420
Tetrahymena pyriformis
?
additional information
enzyme may be involved in the de novo production of platelet-activating factor biosynthesis
646420
Tetrahymena pyriformis
?
-
-
-
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
45
stable
Tetrahymena pyriformis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
microsome and mitochondrion
Tetrahymena pyriformis
9
-
microsome
Tetrahymena pyriformis
Other publictions for EC 3.1.3.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
646420
Tellis
1-O-alkyl-2-acetyl-sn-glycero- ...
Tetrahymena pyriformis
J. Eukaryot. Microbiol.
47
122-128
2000
2
-
-
-
-
-
12
1
5
-
-
1
1
4
-
-
-
-
-
-
4
1
6
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
12
-
1
5
-
-
1
1
-
-
-
-
-
4
1
6
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
646419
Snyder
1-Alkyl-2-acetyl-sn-glycero-3- ...
Rattus norvegicus
Methods Enzymol.
209
230-234
1992
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
646418
Lee
Formation of 1-alkyl-2-acetyl- ...
Rattus norvegicus
J. Biol. Chem.
263
1755-1760
1988
-
-
-
-
-
-
2
1
2
-
-
-
-
2
-
-
-
-
-
5
-
-
1
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
2
-
-
-
-
-
-
-
-
5
-
-
1
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
486103
Lee
A new de novo pathway for the ...
Rattus norvegicus
J. Biol. Chem.
261
5373-5377
1986
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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