BRENDA - Enzyme Database
show all sequences of 3.1.3.58

Characterization of a membrane-regulated sugar phosphate phosphohydrolase from Lactobacillus casei

London, J.; Hausman, S.Z.; Thompson, J.; J. Bacteriol. 163, 951-956 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Fe2+
-
Lactobacillus casei
Zn2+
-
Lactobacillus casei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane
Lactobacillus casei
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
5 mM, divalent cation required for maximal activity, doubles the rate of glucose 6-phosphate hydrolysis
Lactobacillus casei
Mg2+
5 mM, divalent cation required for maximal activity, triples the rate of glucose 6-phosphate hydrolysis
Lactobacillus casei
additional information
Mn2+ and Ni2+ have no effect on enzyme activity
Lactobacillus casei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Lactobacillus casei
62000
-
gel filtration
Lactobacillus casei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glucose 6-phosphate + H2O
Lactobacillus casei
key component of the futile xylitol cycle
D-glucose + phosphate
-
-
?
D-xylitol-5-phosphate + H2O
Lactobacillus casei
relative activity 34%
D-xylitol + phosphate
-
Lactobacillus casei
?
Organism
Organism
UniProt
Commentary
Textmining
Lactobacillus casei
-
Cl-16
-
Lactobacillus casei Cl-16
-
Cl-16
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Lactobacillus casei
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.154
-
2-deoxyglucose 6-phosphate as substrate
Lactobacillus casei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-Deoxy-D-glucose 6-phosphate + H2O
relative activity 75%
646416
Lactobacillus casei
2-Deoxy-D-glucose + phosphate
-
-
-
?
2-Deoxy-D-glucose 6-phosphate + H2O
relative activity 75%
646416
Lactobacillus casei Cl-16
2-Deoxy-D-glucose + phosphate
-
-
-
?
3-phosphoglycerate + H2O
relative activity 4%
646416
Lactobacillus casei
glycerate + phosphate
-
646416
Lactobacillus casei
?
3-phosphoglycerate + H2O
relative activity 4%
646416
Lactobacillus casei Cl-16
glycerate + phosphate
-
646416
Lactobacillus casei Cl-16
?
6-phosphogluconate + H2O
relative activity 69%
646416
Lactobacillus casei
gluconate + phosphate
-
-
-
?
alpha-glycerophosphate + H2O
relative activity 50%
646416
Lactobacillus casei
glycerol + phosphate
-
-
-
?
beta-D-glucose-1,6-diphosphate + H2O
relative activity 56%
646416
Lactobacillus casei
beta-D-glucose + phosphate
-
-
-
?
D-erythrose-4-phosphate + H2O
relative activity 69%
646416
Lactobacillus casei
D-erythrose + phosphate
-
-
-
?
D-fructose-1,6-diphosphate + H2O
relative activity 16%
646416
Lactobacillus casei
D-fructose + phosphate
-
-
-
?
D-fructose-1-phosphate + H2O
relative activity 8%
646416
Lactobacillus casei
D-fructose + phosphate
-
646416
Lactobacillus casei
?
D-fructose-6-phosphate + H2O
relative activity 47%
646416
Lactobacillus casei
D-fructose + phosphate
-
-
-
?
D-galactose-1-phosphate + H2O
relative activity 8%
646416
Lactobacillus casei
D-galactose + phosphate
-
646416
Lactobacillus casei
?
D-galactose-6-phosphate + H2O
relative activity 11%
646416
Lactobacillus casei
D-galactose + phosphate
-
646416
Lactobacillus casei
?
D-glucosamine-6-phosphate + H2O
relative activity 28%
646416
Lactobacillus casei
D-glucosamine + phosphate
-
646416
Lactobacillus casei
?
D-glucose 6-phosphate + H2O
relative activity 100%
646416
Lactobacillus casei
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
key component of the futile xylitol cycle
646416
Lactobacillus casei
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
relative activity 100%
646416
Lactobacillus casei Cl-16
D-glucose + phosphate
-
-
-
?
D-glucose-1-phosphate + H2O
relative activity 3%
646416
Lactobacillus casei
D-glucose + phosphate
-
-
-
?
D-mannose 6-phosphate + H2O
relative activity 80%
646416
Lactobacillus casei
D-mannose + phosphate
-
-
-
?
D-ribitol-5-phosphate + H2O
relative activity 15%
646416
Lactobacillus casei
D-ribitol + phosphate
-
646416
Lactobacillus casei
?
D-ribose 5-phosphate + H2O
relative activity 53%
646416
Lactobacillus casei
D-ribose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
relative activity 53%
646416
Lactobacillus casei Cl-16
D-ribose + phosphate
-
-
-
?
D-sedoheptulose-7-phosphate + H2O
relative activity 34%
646416
Lactobacillus casei
sedoheptulose + phosphate
-
646416
Lactobacillus casei
?
D-sorbitol-6-phosphate + H2O
relative activity 33%
646416
Lactobacillus casei
D-sorbitol + phosphate
-
646416
Lactobacillus casei
?
D-xylitol-5-phosphate + H2O
relative activity 34%
646416
Lactobacillus casei
D-xylitol + phosphate
-
646416
Lactobacillus casei
?
D-xylose-1-phosphate + H2O
relative activity 3%
646416
Lactobacillus casei
D-xylose + phosphate
-
-
-
?
mannitol-1-phosphate + H2O
relative activity 16%
646416
Lactobacillus casei
D-mannitol + phosphate
-
646416
Lactobacillus casei
?
additional information
little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate
646416
Lactobacillus casei
?
-
-
-
-
additional information
little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate
646416
Lactobacillus casei Cl-16
?
-
-
-
-
o-nitrophenyl D-galactose-6-phosphate + H2O
relative activity 6%
646416
Lactobacillus casei
o-nitrophenyl D-galactose + phosphate
-
646416
Lactobacillus casei
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Lactobacillus casei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Lactobacillus casei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Fe2+
-
Lactobacillus casei
Zn2+
-
Lactobacillus casei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane
Lactobacillus casei
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
5 mM, divalent cation required for maximal activity, doubles the rate of glucose 6-phosphate hydrolysis
Lactobacillus casei
Mg2+
5 mM, divalent cation required for maximal activity, triples the rate of glucose 6-phosphate hydrolysis
Lactobacillus casei
additional information
Mn2+ and Ni2+ have no effect on enzyme activity
Lactobacillus casei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
2 * 32000, SDS-PAGE
Lactobacillus casei
62000
-
gel filtration
Lactobacillus casei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glucose 6-phosphate + H2O
Lactobacillus casei
key component of the futile xylitol cycle
D-glucose + phosphate
-
-
?
D-xylitol-5-phosphate + H2O
Lactobacillus casei
relative activity 34%
D-xylitol + phosphate
-
Lactobacillus casei
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Lactobacillus casei
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.154
-
2-deoxyglucose 6-phosphate as substrate
Lactobacillus casei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-Deoxy-D-glucose 6-phosphate + H2O
relative activity 75%
646416
Lactobacillus casei
2-Deoxy-D-glucose + phosphate
-
-
-
?
2-Deoxy-D-glucose 6-phosphate + H2O
relative activity 75%
646416
Lactobacillus casei Cl-16
2-Deoxy-D-glucose + phosphate
-
-
-
?
3-phosphoglycerate + H2O
relative activity 4%
646416
Lactobacillus casei
glycerate + phosphate
-
646416
Lactobacillus casei
?
3-phosphoglycerate + H2O
relative activity 4%
646416
Lactobacillus casei Cl-16
glycerate + phosphate
-
646416
Lactobacillus casei Cl-16
?
6-phosphogluconate + H2O
relative activity 69%
646416
Lactobacillus casei
gluconate + phosphate
-
-
-
?
alpha-glycerophosphate + H2O
relative activity 50%
646416
Lactobacillus casei
glycerol + phosphate
-
-
-
?
beta-D-glucose-1,6-diphosphate + H2O
relative activity 56%
646416
Lactobacillus casei
beta-D-glucose + phosphate
-
-
-
?
D-erythrose-4-phosphate + H2O
relative activity 69%
646416
Lactobacillus casei
D-erythrose + phosphate
-
-
-
?
D-fructose-1,6-diphosphate + H2O
relative activity 16%
646416
Lactobacillus casei
D-fructose + phosphate
-
-
-
?
D-fructose-1-phosphate + H2O
relative activity 8%
646416
Lactobacillus casei
D-fructose + phosphate
-
646416
Lactobacillus casei
?
D-fructose-6-phosphate + H2O
relative activity 47%
646416
Lactobacillus casei
D-fructose + phosphate
-
-
-
?
D-galactose-1-phosphate + H2O
relative activity 8%
646416
Lactobacillus casei
D-galactose + phosphate
-
646416
Lactobacillus casei
?
D-galactose-6-phosphate + H2O
relative activity 11%
646416
Lactobacillus casei
D-galactose + phosphate
-
646416
Lactobacillus casei
?
D-glucosamine-6-phosphate + H2O
relative activity 28%
646416
Lactobacillus casei
D-glucosamine + phosphate
-
646416
Lactobacillus casei
?
D-glucose 6-phosphate + H2O
relative activity 100%
646416
Lactobacillus casei
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
key component of the futile xylitol cycle
646416
Lactobacillus casei
D-glucose + phosphate
-
-
-
?
D-glucose 6-phosphate + H2O
relative activity 100%
646416
Lactobacillus casei Cl-16
D-glucose + phosphate
-
-
-
?
D-glucose-1-phosphate + H2O
relative activity 3%
646416
Lactobacillus casei
D-glucose + phosphate
-
-
-
?
D-mannose 6-phosphate + H2O
relative activity 80%
646416
Lactobacillus casei
D-mannose + phosphate
-
-
-
?
D-ribitol-5-phosphate + H2O
relative activity 15%
646416
Lactobacillus casei
D-ribitol + phosphate
-
646416
Lactobacillus casei
?
D-ribose 5-phosphate + H2O
relative activity 53%
646416
Lactobacillus casei
D-ribose + phosphate
-
-
-
?
D-ribose 5-phosphate + H2O
relative activity 53%
646416
Lactobacillus casei Cl-16
D-ribose + phosphate
-
-
-
?
D-sedoheptulose-7-phosphate + H2O
relative activity 34%
646416
Lactobacillus casei
sedoheptulose + phosphate
-
646416
Lactobacillus casei
?
D-sorbitol-6-phosphate + H2O
relative activity 33%
646416
Lactobacillus casei
D-sorbitol + phosphate
-
646416
Lactobacillus casei
?
D-xylitol-5-phosphate + H2O
relative activity 34%
646416
Lactobacillus casei
D-xylitol + phosphate
-
646416
Lactobacillus casei
?
D-xylose-1-phosphate + H2O
relative activity 3%
646416
Lactobacillus casei
D-xylose + phosphate
-
-
-
?
mannitol-1-phosphate + H2O
relative activity 16%
646416
Lactobacillus casei
D-mannitol + phosphate
-
646416
Lactobacillus casei
?
additional information
little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate
646416
Lactobacillus casei
?
-
-
-
-
additional information
little or no affinity for substrates phosphorylated at the C-1 position, acts on sugars and polyols phosphorylated on the terminal carbon, with a preference for sugars with a D-erythro-configuration, phosphatase activity and substrate specificity are regulated by some components associated with the cytoplasmic membrane, no activity detected with mannose-1-phosphate, maltose-1-phosphate, 2-phosphoglycerate, ribose-1-phosphate, phosphoenolpyruvate, beta-glycerophosphate, phosphothreonine, phosphoserine, and para-nitrophenyl phosphate
646416
Lactobacillus casei Cl-16
?
-
-
-
-
o-nitrophenyl D-galactose-6-phosphate + H2O
relative activity 6%
646416
Lactobacillus casei
o-nitrophenyl D-galactose + phosphate
-
646416
Lactobacillus casei
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32000, SDS-PAGE
Lactobacillus casei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
6
-
Lactobacillus casei
Other publictions for EC 3.1.3.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
655643
Kuznetsova
Enzyme genomics: Application o ...
Thermotoga maritima
FEMS Microbiol. Rev.
29
263-279
2005
-
-
1
-
-
1
-
2
-
4
-
-
-
1
-
-
-
-
-
-
1
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
-
4
-
-
-
-
-
-
-
-
1
1
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
646416
London
Characterization of a membrane ...
Lactobacillus casei, Lactobacillus casei Cl-16
J. Bacteriol.
163
951-956
1985
-
-
-
-
-
-
2
-
1
3
2
2
-
2
-
-
1
-
-
-
1
-
30
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
3
2
2
-
-
-
1
-
-
1
-
30
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-