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Literature summary for 3.1.3.57 extracted from

  • Dollins, D.E.; Xiong, J.P.; Endo-Streeter, S.; Anderson, D.E.; Bansal, V.S.; Ponder, J.W.; Ren, Y.; York, J.D.
    A structural basis for lithium and substrate binding of an inositide phosphatase (2021), J. Biol. Chem., 296, 100059.
    View publication on PubMed
Search for more literature for 3.1.3.57

Cloned(Commentary)

Cloned (Comment) Organism
INPP1 is transfected into Sf9 insect cells via baculovirus infection Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method at 20°C Bos taurus

Protein Variants

Protein Variants Comment Organism
D54A mutation of D54 results in loss of INPP1 activity without significantly changing the substrate affinity. Mutation of a conserved residue that selectively coordinates the putative lithium-binding site results in a dramatic 100fold reduction in the inhibitory constant as compared with wild-type enzyme Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Li+ structural basis for uncompetitive lithium inhibition and substrate recognition. A sequence motif for metal binding within this family of regulatory phosphatases is defined. Lthium preferentially occupies a key site involved in metal-activation only when substrate or product is added. Mutation of a conserved residue that selectively coordinates the putative lithium-binding site results in a dramatic 100fold reduction in the inhibitory constant as compared with wild-type Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 inositol 1,3,4-trisphosphate mutant enzyme D54A, pH and temperature not specified in the publication Bos taurus
0.031
-
 inositol 1,3,4-trisphosphate wild-type enzyme, pH and temperature not specified in the publication Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P21327
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inositol 1,3,4-trisphosphate + H2O
-
 Bos taurus inositol 3,4-bisphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
inositol polyphosphate 1-phosphatase
-
 Bos taurus
INPP1
-
 Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1
-
 Li+ wild-type enzyme, pH and temperature not specified in the publication Bos taurus
107
-
 Li+ mutant enzyme D54A, pH and temperature not specified in the publication Bos taurus