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Literature summary for 3.1.3.57 extracted from

  • Lu, S.Y.; Zhao, G.R.; Wu, A.M.; Jenks, M.A.; Zhang, S.; Liu, J.Y.
    Molecular cloning of a cotton phosphatase gene and its functional characterization (2010), Biochemistry (Moscow), 75, 85-94.
    View publication on PubMed
Search for more literature for 3.1.3.57

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21(DE3), expressed in Saccharomyces cerevisiae JRM4 Gossypium hirsutum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Gossypium hirsutum
K+
-
 Gossypium hirsutum
lithium ion
-
 Gossypium hirsutum
Na+
-
 Gossypium hirsutum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 3'-phosphoadenosine 5'-phosphosulfate KM value lower than 0.01 mM Gossypium hirsutum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration 2mM, 3',5'-bisphosphate nucleotide as substrate Gossypium hirsutum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE Gossypium hirsutum

Organism

Organism UniProt Comment Textmining
Gossypium hirsutum Q8VWZ6 cv. CRI 121
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Gossypium hirsutum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenosine 5'-phosphosulfate + H2O
-
 Gossypium hirsutum ?
-
 ?
3'-phosphoadenosine 5'-phosphosulfate + H2O 128% of activity observed with 3'(2'),5'-bisphosphate nucleotide Gossypium hirsutum adenosine 5'-phosphosulfate + phosphate
-
 ?
3'-phosphoadenosine 5'-phosphosulfate + H2O 128% of activity observed with 3'(2'),5'-bisphosphate nucleotide Gossypium hirsutum CRI 12 adenosine 5'-phosphosulfate + phosphate
-
 ?
adenosine 3',5'-bisphosphate + H2O
-
 Gossypium hirsutum adenosine 5'-phosphate + phosphate
-
 ?
adenosine 3',5'-bisphosphate + H2O
-
 Gossypium hirsutum CRI 12 adenosine 5'-phosphate + phosphate
-
 ?
inositol 1,4,5-trisphosphate + H2O 4.58% of activity observed with 3'(2'),5'-bisphosphate nucleotide Gossypium hirsutum ? + phosphate
-
 ?
inositol 1,4,5-trisphosphate + H2O 4.58% of activity observed with 3'(2'),5'-bisphosphate nucleotide Gossypium hirsutum CRI 12 ? + phosphate
-
 ?
inositol 1,4-bisphosphate + H2O 35% of activity observed with adenosine 3',5'-bisphosphate Gossypium hirsutum ? + phosphate
-
 ?
inositol 1,4-bisphosphate + H2O 35% of activity observed with adenosine 3',5'-bisphosphate Gossypium hirsutum CRI 12 ? + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Gossypium hirsutum

Synonyms

Synonyms Comment Organism
inositol polyphosphate 1-phosphatase dual activities: 3',5'-bisphosphate nucleotidase Gossypium hirsutum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 60 pH 7.5, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 30°C, 2 mM Mg2+, adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
 30°C, pH 7.5, 0.55 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Ca2+
0.05
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Ca2+
0.2
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum lithium ion
0.8
-
 30°C, pH 7.5, 5 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Ca2+
100
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum Na+
200
-
 30°C, pH 7.5, 2 mM Mg2+, 0.2 mM adenosine 3',5'-bisphosphate as substrate Gossypium hirsutum K+