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Literature summary for 3.1.3.56 extracted from
- Molecular and biochemical characterization of three WD-repeat-domain-containing inositol polyphosphate 5-phosphatases in Arabidopsis thaliana (2004), Plant Cell Physiol., 45, 1720-1728.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | wounding and abscisic acid induce the enzyme | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene 5PTase12, expression of His-tagged full length enzyme in yeast | Arabidopsis thaliana |
| gene 5PTase13, expression of His-tagged full length enzyme in yeast | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, maximal activity at 3 mM | Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1D-myo-inositol 1,4,5-trisphosphate + H2O | Arabidopsis thaliana | specific for | 1D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O80560 | gene 5PTase12 | - |
| Arabidopsis thaliana | Q9SYK4 | gene 5PTase13 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from yeast by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | very low expression of 5PTase12 | Arabidopsis thaliana | - |
| leaf | very low expression of 5PTase13 | Arabidopsis thaliana | - |
| additional information | expression pattern | Arabidopsis thaliana | - |
| additional information | expression pattern, no expression in root | Arabidopsis thaliana | - |
| root | low expression of 5PTase12 | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| shoot tip | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
| stem | low expression of 5PTase13 | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1D-myo-inositol 1,4,5-trisphosphate + H2O | specific for | Arabidopsis thaliana | 1D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
| 1D-myo-inositol 1,4,5-trisphosphate + H2O | absolutely specific for | Arabidopsis thaliana | 1D-myo-inositol 1,4-bisphosphate + phosphate | - |
? | |
| additional information | substrate specificity, no activity with 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate, 1D-myo-inositol 1,3,4,5-tetrakisphosphate, 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate, 1-phosphatidyl-1D-myo-inositol 5-phosphate, 1-phosphatidyl-1D-myo-inositol 4-phosphate, and 1-phosphatidyl-1D-myo-inositol 3-phosphate | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| additional information | the enzyme contains a WD-repeat domain and a 5PTase catalytic domain | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5PTase | - |
Arabidopsis thaliana |
| inositol polyphosphate 5-phosphatase | - |
Arabidopsis thaliana |
| type II inositol polyphosphate 5-phosphatase | - |
Arabidopsis thaliana |
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