Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.56 extracted from

  • Communi, D.; Lecocq, R.; Erneux, C.
    Arginine 343 and 350 are two active site residues involved in substrate binding by human type I D-myo-inositol 1,4,5-trisphosphate 5-phosphatase (1996), J. Biol. Chem., 271, 11676-11683.
    View publication on PubMed
Search for more literature for 3.1.3.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HB 101 host bacteria Homo sapiens

Protein Variants

Protein Variants Comment Organism
R343A increased Km and dramatic loss of activity Homo sapiens
R350A increased Km Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,3-bisphosphoglycerate
-
 Homo sapiens
Phenylglyoxal Arg-343 and Arg-350 were found to be covalently modified; irreversible, substrates and 2,3-bisphosphoglycerate protect against inactivation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 D-myo-inositol 1,4,5-trisphosphate native enzyme Homo sapiens
0.205
-
 D-myo-inositol 1,4,5-trisphosphate R343A mutant Homo sapiens
0.32
-
 D-myo-inositol 1,4,5-trisphosphate R350A mutant Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 SDS-PAGE, type I enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1,4,5-trisphosphate + H2O Homo sapiens regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme using His-tag Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
 Vmax, R343A mutant Homo sapiens
191
-
 Vmax, native enzyme Homo sapiens
237
-
 Vmax, R350A mutant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?