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Literature summary for 3.1.3.56 extracted from

  • Mitchell, C.A.; Connolly, T.M.; Majerus, P.W.
    Identification and isolation of a 75-kDa inositol polyphosphate-5-phosphatase from human platelets (1989), J. Biol. Chem., 264, 8873-8877.
    View publication on PubMed
Search for more literature for 3.1.3.56

Inhibitors

Inhibitors Comment Organism Structure
Inositol 1,3,4,5-tetrakisphosphate
-
 Homo sapiens
inositol 1,4-bisphosphate
-
 Homo sapiens
Mg2+ above 3 mM Homo sapiens
phosphate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 D-myo-Inositol 1,3,4,5-tetrakisphosphate
-
 Homo sapiens
0.016 0.019 D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Homo sapiens
Mg2+ required, maximum activity with 1-3 mM, inhibitory above 3 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O Homo sapiens
-
 D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O Homo sapiens
-
 D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein 75000 Da enzyme not phosphorylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
platelet
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O very poor substrate Homo sapiens D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
inositol 1,2-cyclic 4,5-trisphosphate + H2O poor substrate Homo sapiens ?
-
 ?
additional information glycerophosphoinositol 4,5-bisphosphate is not hydrolyzed Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75000, gel filtration, SDS-PAGE Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
 Inositol 1,3,4,5-tetrakisphosphate 45000 Da enzyme Homo sapiens
0.015
-
 Inositol 1,3,4,5-tetrakisphosphate 75000 Da enzyme Homo sapiens