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Literature summary for 3.1.3.56 extracted from

  • Erneux, C.; Lemos, M.; Verjans, B.; Vanderhaeghen, P.; Delvaux, A.; Dumont, J.E.
    Soluble and particulate Ins(1,4,5)P3/Ins(1,3,4,5)P4 5-phosphatase in bovine brain (1989), Eur. J. Biochem., 181, 317-322.
    View publication on PubMed
Search for more literature for 3.1.3.56

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 D-myo-inositol 1,4,5-trisphosphate pH 7.4, 37°C Bos taurus
0.011
-
 D-myo-Inositol 1,3,4,5-tetrakisphosphate pH 7.4, 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane 85-90% of total activity, can be solubilized by cholate or to lesser extent by 2 M KCl Bos taurus 16020
-
soluble 10-15% of total activity Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration without cholate, type I enzyme Bos taurus
48000
-
 SDS-PAGE, type I enzyme Bos taurus
51000
-
 gel filtration in presence of 0.9% cholate, type I enzyme Bos taurus
52000
-
 gel filtration in presence of 0.9% cholate, type II enzyme Bos taurus
115000
-
 gel filtration without cholate, type II enzyme Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O Bos taurus
-
 D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O Bos taurus regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of membrane bound and soluble enzyme Bos taurus
type I enzyme Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
 purified type I enzyme, pH 7.4, 37°C Bos taurus

Storage Stability

Storage Stability Organism
-80°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O substrate for type I but not type II enzyme Bos taurus D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Bos taurus D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Bos taurus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Bos taurus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?