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Literature summary for 3.1.3.56 extracted from

  • Downes, C.P.; Mussat, M.C.; Michell, R.H.
    The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane (1982), Biochem. J., 203, 169-177.
    View publication on PubMed
Search for more literature for 3.1.3.56

Inhibitors

Inhibitors Comment Organism Structure
2,3-bisphosphoglycerate competitive with D-myo-inositol 1,4,5-trisphosphate Homo sapiens
EDTA no activity in presence of 1 mM EDTA Homo sapiens
Glycerophosphoinositol 4,5-bisphosphate competitive with D-myo-inositol 1,4,5-trisphosphate Homo sapiens
Glycerophosphoinositol 4-phosphate
-
 Homo sapiens
Mg2+ above 12 mM Homo sapiens
additional information inositol 1,4-bisphosphate does not inhibit enzyme activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 D-myo-inositol 1,4,5-trisphosphate pH 7.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane two isoforms postulated one of which is membrane associated Homo sapiens 16020
-
soluble two isoforms postulated one of which is soluble Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Homo sapiens
Mg2+ required, maximum activity with 1-5 mM, inhibitory above 12 mM Homo sapiens
Mn2+ can partially replace Mg2+, 50% of activity with Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-myo-inositol 1,4,5-trisphosphate + H2O Homo sapiens
-
 D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte ghosts Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0028
-
 Vmax, pH 7.0, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
glycerophosphoinositol 4,5-bisphosphate + H2O at 13% of the rate of inositol 1,4,5-trisphosphate hydrolysis Homo sapiens glycerophosphoinositol 4-phosphate + phosphate
-
 ?
additional information glycerophosphoinositol 4-phosphate and inositol 1,4-bisphosphate are not hydrolyzed Homo sapiens ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.35
-
 2,3-bisphosphoglycerate
-
 Homo sapiens