BRENDA - Enzyme Database
show all sequences of 3.1.3.54

Characterization of yeast fructose-2,6-bisphosphate 6-phosphatase

Plankert, U.; Purwin, C.; Holzer, H.; FEBS Lett. 239, 69-72 (1988)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-Fructose 2-phosphate
50% inhibition at 0.008 mM
Saccharomyces cerevisiae
D-fructose 6-phosphate
50% inhibition at 0.08 mM
Saccharomyces cerevisiae
D-glucose 6-phosphate
50% inhibition at 0.045 mM
Saccharomyces cerevisiae
additional information
no inhibitor: fructose
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0072
-
D-fructose 2,6-bisphosphate
pH 6.0
Saccharomyces cerevisiae
0.079
-
D-fructose 2,6-bisphosphate
pH 7.0
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose 2,6-bisphosphate + H2O
Saccharomyces cerevisiae
participates in maintenance of a steady state level of fructose 2,6-bisphosphate
D-fructose 2-phosphate + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose 2,6-bisphosphate + H2O
-
643865
Saccharomyces cerevisiae
D-fructose 2-phosphate + phosphate
-
-
-
?
D-fructose 2,6-bisphosphate + H2O
participates in maintenance of a steady state level of fructose 2,6-bisphosphate
643865
Saccharomyces cerevisiae
D-fructose 2-phosphate + phosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
D-fructose 2,6-bisphosphate
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-Fructose 2-phosphate
50% inhibition at 0.008 mM
Saccharomyces cerevisiae
D-fructose 6-phosphate
50% inhibition at 0.08 mM
Saccharomyces cerevisiae
D-glucose 6-phosphate
50% inhibition at 0.045 mM
Saccharomyces cerevisiae
additional information
no inhibitor: fructose
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0072
-
D-fructose 2,6-bisphosphate
pH 6.0
Saccharomyces cerevisiae
0.079
-
D-fructose 2,6-bisphosphate
pH 7.0
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-fructose 2,6-bisphosphate + H2O
Saccharomyces cerevisiae
participates in maintenance of a steady state level of fructose 2,6-bisphosphate
D-fructose 2-phosphate + phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose 2,6-bisphosphate + H2O
-
643865
Saccharomyces cerevisiae
D-fructose 2-phosphate + phosphate
-
-
-
?
D-fructose 2,6-bisphosphate + H2O
participates in maintenance of a steady state level of fructose 2,6-bisphosphate
643865
Saccharomyces cerevisiae
D-fructose 2-phosphate + phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
D-fructose 2,6-bisphosphate
Saccharomyces cerevisiae
Other publictions for EC 3.1.3.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643864
Plankert
Yeast fructose-2,6-bisphosphat ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae M1
Eur. J. Biochem.
196
191-196
1991
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-
-
-
-
-
2
-
1
2
-
2
-
3
-
1
1
-
-
-
1
-
4
-
1
-
4
-
3
1
-
-
-
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-
-
-
-
-
-
-
-
-
2
-
-
1
2
-
2
-
-
1
1
-
-
1
-
4
-
1
-
4
-
3
1
-
-
-
-
-
-
-
-
643865
Plankert
Characterization of yeast fruc ...
Saccharomyces cerevisiae
FEBS Lett.
239
69-72
1988
-
-
-
-
-
-
4
2
-
-
-
1
-
2
-
-
-
-
-
-
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2
-
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-
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-
1
-
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-
4
-
2
-
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-
1
-
-
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-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
643862
Purwin
Fructose 2-phosphate, an inter ...
Saccharomyces cerevisiae
Eur. J. Biochem.
164
27-30
1987
-
-
-
-
-
-
2
1
-
1
2
1
-
2
-
-
1
-
-
-
1
-
3
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
2
1
-
-
-
1
-
-
1
-
3
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
643863
Purwin
Fructofuranose 2-phosphate is ...
Saccharomyces cerevisiae
Eur. J. Biochem.
165
543-545
1987
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-
-
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-
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-
-
1
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2
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2
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1
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2
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