Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.53 extracted from

  • Scotto-Lavino, E.; Garcia-Diaz, M.; Du, G.; Frohman, M.A.
    Basis for the isoform-specific interaction of myosin phosphatase subunits protein phosphatase 1c beta and myosin phosphatase targeting subunit 1 (2010), J. Biol. Chem., 285, 6419-6424.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.53

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in CHO cells using a retroviral vector Homo sapiens

Protein Variants

Protein Variants Comment Organism
A233S site-directed mutagenesis of subunit PP1cgamma Homo sapiens
A281G site-directed mutagenesis of subunit PP1cgamma Homo sapiens
F38A site-directed mutagenesis of subunit MYPT1, the mutant subunit is unable to bind to subunit PP1cbeta Homo sapiens
G280A site-directed mutagenesis of subunit PP1cbeta, the mutation does not affect interaction with MYPT1 Homo sapiens
H237N/K238R site-directed mutagenesis of subunit PP1cgamma, the mutant subunit shows over 90% reduced ability to bind to subunit MYPT1 Homo sapiens
N236H/R237K site-directed mutagenesis of subunit PP1cbeta, the mutation does not affect interaction with MYPT1 Homo sapiens
Q198T site-directed mutagenesis of subunit PP1cgamma, mutant subunit is unable to bind to subunit MYPT1 Homo sapiens
S232A site-directed mutagenesis of subunit PP1cbeta, the mutation does not affect interaction with MYPT1 Homo sapiens
T197Q site-directed mutagenesis of subunit PP1cbeta, the mutant subunit is unable to bind to subunit MYPT1 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Subunits

Subunits Comment Organism
More myosin phosphatase is a complex that consists of a catalytic subunit protein phosphatase 1c, PP1cbeta, a large subunit myosin phosphatase targeting subunit, MYPT, and a small subunit. Stability of PP1cbeta and MYPT1 is interdependent, knocking down one of the subunits decreases the expression level of the other. MYPT1 binding is restricted to PP1cbeta, the central region of PP1cbeta confers the isoform-specific binding, while the variable, C-terminal domain of PP1cbeta is the region key for isoform-specific interaction with MYPT1. Sequence comparison of PP1 isoforms, overview Homo sapiens

Synonyms

Synonyms Comment Organism
myosin phosphatase
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens stability of subunits PP1cbeta and MYPT1 is interdependent, knocking down one of the subunits decreases the expression level of the other down

General Information

General Information Comment Organism
additional information myosin II association with actin, which triggers contraction, is regulated by orchestrated waves of phosphorylation/dephosphorylation of the myosin regulatory light chain. Dephosphorylation is mediated by myosin phosphatase Homo sapiens