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Literature summary for 3.1.3.53 extracted from

  • Okamoto, R.; Kato, T.; Mizoguchi, A.; Takahashi, N.; Nakakuki, T.; Mizutani, H.; Isaka, N.; Imanaka-Yoshida, K.; Kaibuchi, K.; Lu, Z.; Mabuchi, K.; Tao, T.; Hartshorne, D.J.; Nakano, T.; Ito, M.
    Characterization and function of MYPT2, a target subunit of myosin phosphatase in heart (2006), Cell. Signal., 18, 1408-1416.
    View publication on PubMed
Search for more literature for 3.1.3.53

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of MYPT1 and MYPT2 in primary cultures of cardiac myocytes and in COS7 cells using baculoviral and adenoviral expression systems Mammalia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00103
-
 myosin light-chain phosphate smooth muscle, type 1 protein phosphatase delta and MYPT1 Mammalia
0.00136
-
 myosin light-chain phosphate smooth muscle, type 1 protein phosphatase delta and MYPT2 Mammalia
0.00394
-
 myosin light-chain phosphate smooth muscle, type 1 protein phosphatase delta only Mammalia
0.00502
-
 myosin light-chain phosphate cardiac muscle, type 1 protein phosphatase delta and MYPT1 Mammalia
0.00551
-
 myosin light-chain phosphate cardiac muscle, type 1 protein phosphatase delta and MYPT2 Mammalia
0.01117
-
 myosin light-chain phosphate cardiac muscle, type 1 protein phosphatase delta only Mammalia

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information close to the Z-line, the A-Band and mitochondrion Mammalia
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 Western blot analysis Mammalia

Organism

Organism UniProt Comment Textmining
Mammalia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the native and recombinant proteins by immunoprecipitation Mammalia

Source Tissue

Source Tissue Comment Organism Textmining
cardiac muscle
-
 Mammalia
-
smooth muscle
-
 Mammalia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myosin light-chain phosphate + H2O
-
 Mammalia myosin light-chain + phosphate
-
 ?

Subunits

Subunits Comment Organism
More enzyme has 3 subunits, the catalytic subunit type 1 protein phosphatase delta, the regulatory subunit MYPT1 in smooth muscle and MYPT 2 an isoform of MYPT1 in striated muscle Mammalia

Synonyms

Synonyms Comment Organism
MP
-
 Mammalia
myosin phosphatase
-
 Mammalia
[myosin-light-chain] phosphatase
-
 Mammalia