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Literature summary for 3.1.3.53 extracted from
- Myosin assembly critical for the enzyme activity of smooth muscle myosin phosphatase: Effects of MgATP, ionic strength, and Mg2+ (2001), J. Biochem., 129, 881-889.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | P-myosin assembly is essential for SMMP activity | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | inhibits SMMP activity, dissociates P-myosin primarily into an extended conformation | Gallus gallus |  |
| Mg2+ | above 5 mM | Gallus gallus | |
| MgATP2- | inhibits SMMP activity towards P-myosin, disassembles P-myosin mostly into a folded conformation | Gallus gallus | |
| additional information | increases in the ionic strength inhibit SMMP activity | Gallus gallus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00045 | - |
Phosphorylated myosin | at a ionic strength of 0.15 M | Gallus gallus | |
| 0.0024 | - |
Phosphorylated myosin | at a ionic strength of 0.15 M, in the presence of 0.1 mM MgATP | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gizzard | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myosin light-chain phosphate + H2O | from bovine stomach | Gallus gallus | myosin light-chain + phosphate | - |
? | |
| myosin light-chain phosphate + H2O | 20 kDa myosin light chain | Gallus gallus | myosin light-chain + phosphate | - |
? | |
| phosphorylated myosin + H2O | from bovine stomach, P-myosin assembly is essential for SMMP activity | Gallus gallus | myosin + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Gallus gallus |
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Release 2023.1 (January 2023)
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