Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.52 extracted from

  • Dolatabad, M.; Guo, L.; Xiao, P.; Zhu, Z.; He, Q.; Yang, D.; Qu, C.; Guo, S.; Fu, X.; Li, R.; Ge, L.; Hu, K.; Liu, H.; Shen, Y.; Yu, X.; Sun, J.; Zhang, P.
    Crystal structure and catalytic activity of the PPM1K N94K mutant (2019), J. Neurochem., 148, 550-560 .
    View publication on PubMed
Search for more literature for 3.1.3.52

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant N94K, three Mg2+ ions are located in the active site of mutant N94K instead of two Mg2+ ions in the PPM1K wild type Homo sapiens

Protein Variants

Protein Variants Comment Organism
N94K missense mutant due to single nucleotide polymorphism. Mutation significantly impairs Mg2+-dependent catalytic activity and induces a conformational change in the key residue in coordinating the Mg2+ in the active site Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8
-
 4-nitrophenyl phosphate mutant N94K, presence of Mn2+, 30°C, pH 7.5 Homo sapiens
4.8
-
 4-nitrophenyl phosphate wild-type, presence of Mn2+, 30°C, pH 7.5 Homo sapiens
39.8
-
 4-nitrophenyl phosphate mutant N94K, presence of Mg2+, 37°C, pH 7.5 Homo sapiens
50.9
-
 4-nitrophenyl phosphate wild-type, presence of Mg2+, 37°C, pH 7.5 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ two Mg2+ ions are located in the active site of wild-type Homo sapiens
Mn2+ Mn2+ may substitute for Mg2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N3J5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Homo sapiens 4-nitrophenol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PP2Cm
-
 Homo sapiens
PPM1K
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 4-nitrophenyl phosphate mutant N94K, presence of Mg2+, 37°C, pH 7.5 Homo sapiens
1
-
 4-nitrophenyl phosphate wild-type, presence of Mg2+, 37°C, pH 7.5 Homo sapiens
22.9
-
 4-nitrophenyl phosphate mutant N94K, presence of Mn2+, 30°C, pH 7.5 Homo sapiens
40.9
-
 4-nitrophenyl phosphate wild-type, presence of Mn2+, 30°C, pH 7.5 Homo sapiens