Cloned (Comment) | Organism |
---|---|
expressed in HEK293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A193X | single-nucleotide polymorphisms (SNPs) in human PP2Cm: mutant demonstrate basal activity against branched-chain alpha-keto dehydrogenase (BCKD) but impaired responsiveness to branched-chain-alpha-keto acids (BCKAs) | Homo sapiens |
E321K | single-nucleotide polymorphisms (SNPs) in human PP2Cm: mutant demonstrate basal activity against branched-chain alpha-keto dehydrogenase (BCKD) but impaired responsiveness to branched-chain-alpha-keto acids (BCKAs). Mutant is not detected by immunoblot using human PP2Cm antibody. Therefore, it remains unclear whether this mutant is phosphatase dead due to loss-of-function mutations or unstable due to premature truncation | Homo sapiens |
F359X | single-nucleotide polymorphisms (SNPs) in human PP2Cm: frameshift mutant shows no phosphatase activity at basal or after branched-chain-alpha-keto acids (BCKA) treatment. Mutant is not detected by immunoblot using human PP2Cm antibody. Therefore, it remains unclear whether this mutant is phosphatase dead due to loss-of-function mutations or unstable due to premature truncation | Homo sapiens |
I167T | single-nucleotide polymorphisms (SNPs) in human PP2Cm: mutation has no an elevated activity compared to wild-type | Homo sapiens |
additional information | motifs responsible for PP2Cm and E2 interaction: using deletion mutants it is shown that the region between the residues 46 and 61 is critical to the association of PP2Cm with the complex | Mus musculus |
N94K | single-nucleotide polymorphisms (SNPs) in human PP2Cm: mutation has no impact on PP2Cm activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | PP2Cm interacts with the BCKD E2 subunit and competes with the BCKD kinase in a substrate-dependent and mutually exclusive manner | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N3J5 | - |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
diaphragm | - |
Mus musculus | - |
heart | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
skeletal muscle | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PP2Cm interacts with the BCKD E2 subunit and competes with the BCKD kinase in a substrate-dependent and mutually exclusive manner | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PP2Cm | - |
Mus musculus |
PP2Cm | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Mus musculus | skeletal muscle | down |
Mus musculus | branched-chain amino acid (BCAA) controls PP2Cm gene transcription | additional information |
Mus musculus | PP2Cm expression is highly enriched in brain, heart, liver, kidney and diaphragm | up |