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BRENDA support

Literature summary for 3.1.3.52 extracted from

  • Damuni, Z.; Merryfield, M.L.; Humphreys, J.S.; Reed, L.J.
    Purification and properties of branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney (1984), Proc. Natl. Acad. Sci. USA, 81, 4335-4338.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
poly(L-arginine) stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus
poly(L-lysine) stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus
protamine stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acyl-CoA inhibition is partially reversed by Mg2+ Bos taurus
ADP 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ Bos taurus
ATP 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ Bos taurus
CDP 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ Bos taurus
CoA inhibition is not reversed by Mg2+ Bos taurus
CTP 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ Bos taurus
GDP 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ Bos taurus
GTP 50% inhibition at 60 mM; inhibition is completely reversed by Mg2+ Bos taurus
guanosine 5'-(beta,gamma-imido)triphosphate
-
Bos taurus
heparin 50% inhibition at 0.002 mg/ml, partially reversed by Mg2+ Bos taurus
additional information little inhibitory effect: AMP; little inhibitory effect: GMP; little inhibitory effect: NAD+; little inhibitory effect: NADH; little inhibitory effect: UMP Bos taurus
UDP 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ Bos taurus
UTP 50% inhibition at 100 mM; inhibition is completely reversed by Mg2+ Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information active in absence of divalent cation Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
230000
-
gel filtration, under conditions of high dilution, low MW form Bos taurus
460000
-
gel filtration Bos taurus
460000
-
high MW form Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O Bos taurus
-
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
a high and a low MW form Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.24
-
-
Bos taurus

Storage Stability

Storage Stability Organism
4°C, partially purified enzyme stable for several weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O
-
Bos taurus [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.3
-
Bos taurus