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Literature summary for 3.1.3.52 extracted from

  • Damuni, Z.; Reed, L.J.
    Branched-chain alpha-keto acid dehydrogenase phosphatase and its inhibitor protein from bovine kidney (1988), Methods Enzymol., 166, 321-329.
    View publication on PubMed
Search for more literature for 3.1.3.52

Activating Compound

Activating Compound Comment Organism Structure
histone H3 stimulates at 0.036 mg/ml Bos taurus
poly(L-arginine) stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus
poly(L-lysine) stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus
protamine stimulates 1.5-3fold at 0.0036 mg/ml Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acyl-CoA
-
 Bos taurus
ADP 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ Bos taurus
ATP 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ Bos taurus
CDP 50% inhibition at 400 mM; inhibition is completely reversed by Mg2+ Bos taurus
CoA
-
 Bos taurus
CTP 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ Bos taurus
GDP 50% inhibition at 200 mM; inhibition is completely reversed by Mg2+ Bos taurus
GTP 50% inhibition at 60 mM; inhibition is completely reversed by Mg2+ Bos taurus
guanosine 5'-(beta,gamma-imido)triphosphate
-
 Bos taurus
heparin
-
 Bos taurus
Inhibitor protein purification of heat- and acid-stable inhibitor protein, which regulates activity Bos taurus
additional information little inhibitory effect: AMP; little inhibitory effect: GMP; little inhibitory effect: NAD+; little inhibitory effect: NADH; little inhibitory effect: UMP Bos taurus
nucleoside diphosphates inhibition is completely reversed by Mg2+ Bos taurus
nucleoside triphosphates inhibition is completely reversed by Mg2+ Bos taurus
UDP 50% inhibition at 250 mM; inhibition is completely reversed by Mg2+ Bos taurus
UTP 50% inhibition at 100 mM; inhibition is completely reversed by Mg2+ Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion inner membrane-matrix compartment Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information active in absence of divalent cation Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
460000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O Bos taurus
-
 [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.158
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O
-
 Bos taurus [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate
-
 ?

Subunits

Subunits Comment Organism
More enzyme is associated with the 3-methyl-2-oxobutanoate dehydrogenase complex Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000013
-
 Inhibitor protein pH 7.3, 30°C Bos taurus