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Literature summary for 3.1.3.52 extracted from
- Purification and properties of the catalytic subunit of the branched-chain alpha-keto acid dehydrogenase phosphatase from bovine kidney mitochondria (1987), J. Biol. Chem., 262, 5129-5132.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Bos taurus |  |
| ADP | inhibition is completely reversed by Mg2+; inhibition is completely reversed by spermine | Bos taurus | |
| ATP | completely reversed by spermine; inhibition is completely reversed by Mg2+ | Bos taurus | |
| CoA | - |
Bos taurus | |
| GDP | inhibition is completely reversed by Mg2+; inhibition is completely reversed by spermine | Bos taurus | |
| GTP | inhibition is completely reversed by Mg2+; inhibition is completely reversed by spermine | Bos taurus | |
| Inhibitor protein | BCKDH phosphatase inhibitor protein, inhibits at nanomolar concentrations | Bos taurus | |
| isobutyryl-CoA | - |
Bos taurus | |
| isovaleryl-CoA | - |
Bos taurus | |
| additional information | little inhibitory effect: nucleoside monophosphates; little inhibitory effect: protein phosphatase inhibitor 1 and inhibitor 2 | Bos taurus | |
| nucleoside diphosphates | - |
Bos taurus | |
| nucleoside triphosphates | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
1 * 33000, catalytic subunit, SDS-PAGE | Bos taurus |
| 460000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O | Bos taurus | - |
[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| catalytic subunit | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.328 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 6 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | inactive with phosphorylase a and with 4-nitrophenyl phosphate | Bos taurus | ? | - |
? | |
| [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] phosphate + H2O | - |
Bos taurus | [3-methyl-2-oxobutanoate dehydrogenase (lipoamide)] + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | 1 * 33000, catalytic subunit, SDS-PAGE | Bos taurus |
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