BRENDA - Enzyme Database
show all sequences of 3.1.3.51

Enzymatic dephosphorylation of endogenous and exogenous dolichyl monophosphate by calf brain membranes

Burton, W.A.; Scher, M.G.; Waechter, C.J.; Arch. Biochem. Biophys. 208, 409-417 (1981)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
CaCl2
Bos taurus
MgCl2
weak inhibition
Bos taurus
Mn2+
10 mM, 57% inhibition
Bos taurus
NaF
10 mM, 82% loss of activity
Bos taurus
retinyl monophosphate
-
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
dolichyl phosphate
-
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plasma membrane
-
Bos taurus
5886
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
calf
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl phosphate + H2O
-
134914
Bos taurus
dolichol + phosphate
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
3 min, 2% loss of activity
Bos taurus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
pH 5: about 35% of maximal activity, pH 9: about 50% of maximal activity
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
CaCl2
Bos taurus
MgCl2
weak inhibition
Bos taurus
Mn2+
10 mM, 57% inhibition
Bos taurus
NaF
10 mM, 82% loss of activity
Bos taurus
retinyl monophosphate
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
dolichyl phosphate
-
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plasma membrane
-
Bos taurus
5886
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl phosphate + H2O
-
134914
Bos taurus
dolichol + phosphate
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
3 min, 2% loss of activity
Bos taurus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
pH 5: about 35% of maximal activity, pH 9: about 50% of maximal activity
Bos taurus
Other publictions for EC 3.1.3.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
134922
Frank
Purification and characterizat ...
Bos taurus, Sus scrofa
J. Biol. Chem.
273
11791-11798
1998
-
-
-
-
-
-
10
2
2
1
1
-
-
4
-
-
1
-
-
3
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
2
2
1
1
-
-
-
-
1
-
3
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134921
Bhat
Developmental changes in enzym ...
Rattus norvegicus
J. Neurochem.
56
339-344
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134913
Sumbilla
Dolichol kinase, phosphatase, ...
Bos taurus
Methods Enzymol.
111
471-482
1988
1
-
-
-
-
-
4
1
2
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
2
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134906
Ravi
-
Submicrosomal distribution of ...
Glycine max
Biochim. Biophys. Acta
884
497-501
1986
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134911
Keller
Characterization of polyisopre ...
Rattus norvegicus
Arch. Biochem. Biophys.
249
207-214
1986
-
-
-
-
-
-
6
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134912
Scher
Dolichyl phosphate metabolism ...
Sus scrofa
J. Biol. Chem.
260
13742-13746
1985
-
-
-
-
-
-
-
2
1
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134915
Boscoboinik
Dolichyl phosphate phosphatase ...
Rattus norvegicus
Biochim. Biophys. Acta
794
41-48
1984
9
-
-
-
-
-
4
-
2
-
1
-
-
2
-
-
-
-
-
2
-
1
1
-
2
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
4
1
-
2
-
1
-
-
-
-
-
-
2
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
134916
Rip
The submicrosomal distribution ...
Rattus norvegicus
J. Biol. Chem.
258
14926-14930
1983
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134920
Ravi
Characterization of dolichol a ...
Glycine max
Biochem. J.
213
513-518
1983
3
-
-
-
-
-
4
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
4
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
134917
Belocopitow
Dolichyl-phosphate phosphatase ...
Rattus norvegicus
Eur. J. Biochem.
125
167-173
1982
-
-
-
-
-
-
8
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134918
Rupar
The subcellular localization o ...
Rattus norvegicus
J. Biol. Chem.
257
3090-3094
1982
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134910
Rip
Localization of a dolichyl pho ...
Rattus norvegicus
J. Biol. Chem.
256
1929-1934
1981
-
-
-
-
-
-
5
1
2
1
-
-
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
2
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
134914
Burton
Enzymatic dephosphorylation of ...
Bos taurus
Arch. Biochem. Biophys.
208
409-417
1981
-
-
-
-
-
-
5
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
134919
Adrian
Dolichyl phosphate phosphatase ...
Tetrahymena pyriformis
Biochem. J.
197
233-238
1981
-
-
-
-
-
-
6
-
3
1
2
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
3
1
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134908
Wedgwood
Enzymatic activities in cultur ...
Homo sapiens
J. Biol. Chem.
255
1120-1123
1980
-
-
-
-
-
-
1
1
1
-
-
-
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
134909
Idoyaga-Vargas
A phosphatase acting on dolich ...
Rattus norvegicus
FEBS Lett.
112
63-66
1980
1
-
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134907
Adrian
A dolichyl phosphate-cleaving ...
Tetrahymena pyriformis, Tetrahymena pyriformis WH14
Biochim. Biophys. Acta
575
431-438
1979
-
-
-
-
-
-
3
-
-
1
-
2
-
3
-
-
-
-
-
-
-
1
6
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
2
-
-
-
-
-
-
-
1
6
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-