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Literature summary for 3.1.3.5 extracted from

  • Skladanowski, A.C.; Newby, A.C.
    Partial purification and properties of an AMP-specific 5'-nucleotidase from pigeon heart (1990), Biochem. J., 268, 117-122.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.5

Activating Compound

Activating Compound Comment Organism Structure
ADP activation of cytosolic enzyme Columba livia
ATP activation Columba livia

Application

Application Comment Organism
pharmacology potential site for pharmacological intervention to protect organs against nucleotide depletion Columba livia

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl phosphate
-
 Columba livia
adenosine 5'-(alpha,beta-methylene)diphosphate not inhibitory Columba livia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.7
-
 5'-AMP
-
 Columba livia
7.7
-
 5'-IMP
-
 Columba livia

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Columba livia 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation Columba livia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Columba livia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-IMP + H2O Columba livia the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Columba livia
-
 pigeon
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Columba livia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.8
-
-
 Columba livia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Columba livia adenosine + phosphate
-
 ?
5'-CMP + H2O 61.7% of the activity with 5'-AMP Columba livia cytidine + phosphate
-
 ?
5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Columba livia deoxyadenosine + phosphate
-
 ?
5'-dAMP + H2O 66% of the activity with 5'-AMP Columba livia deoxyadenosine + phosphate
-
 ?
5'-dGMP + H2O i.e. 2'-deoxyguanosine 5'-phosphate Columba livia deoxyguanosine + phosphate
-
 ?
5'-dGMP + H2O 49.9% of the activity with 5'-AMP Columba livia deoxyguanosine + phosphate
-
 ?
5'-dIMP + H2O i.e. 2'-deoxyinosine 5'-phosphate Columba livia deoxyinosine + phosphate
-
 ?
5'-dIMP + H2O 60.9% of the activity with 5'-AMP Columba livia deoxyinosine + phosphate
-
 ?
5'-GMP + H2O 33.5% of the activity with 5'-AMP Columba livia guanosine + phosphate
-
 ?
5'-IMP + H2O 33.1% of the activity with 5'-AMP Columba livia inosine + phosphate
-
 ?
5'-IMP + H2O the enzyme is principally responsible for dephosphorylation of 5'-IMP but may have some role in adenosine formation Columba livia ?
-
 ?
5'-TMP + H2O 15.2% of the activity with 5'-AMP Columba livia thymidine + phosphate
-
 ?
5'-UMP + H2O 49.5% of the activity with 5'-AMP Columba livia uridine + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Columba livia