Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
agglutinin | enzyme from glioblastoma cells | Rattus norvegicus | |
ATP | - |
Rattus norvegicus | |
Co2+ | strong, enzyme from glioblastoma cells | Rattus norvegicus | |
concanavalin A | enzyme from glioblastoma cells | Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
Mn2+ | strong, enzyme from glioblastoma cells | Rattus norvegicus | |
Ni2+ | strong, enzyme from glioblastoma cells | Rattus norvegicus | |
Zn2+ | strong, enzyme from glioblastoma cells | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
5'-IMP | enzyme from glioblastoma cells | Rattus norvegicus | |
0.02 | - |
5'-AMP | enzyme from glioblastoma cells | Rattus norvegicus | |
0.031 | - |
5'-UMP | enzyme from glioblastoma cells | Rattus norvegicus | |
0.038 | - |
5'-GMP | enzyme from glioblastoma cells | Rattus norvegicus | |
0.07 | - |
5'-CMP | enzyme from glioblastoma cells | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Rattus norvegicus | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure | Rattus norvegicus |
74000 | - |
4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure | Rattus norvegicus |
135000 | - |
enzyme from glioblastoma cells, gel filtration in presence of 0.6% sodium deoxycholate | Rattus norvegicus |
268000 | - |
enzyme from glioblastoma cells, gel filtration in presence of 0.1% Triton X-100 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glioblastoma cells, membrane-bound | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
glioblastoma cell | Rugli cells | Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, enzyme from glioblastoma cells, 40 mM Tris-HCl buffer, pH 7.4, 0.1 M NaCl, 0.1% Triton X-100, several months stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-AMP + H2O | - |
Rattus norvegicus | adenosine + phosphate | - |
? | |
5'-CMP + H2O | - |
Rattus norvegicus | cytidine + phosphate | - |
? | |
5'-GMP + H2O | - |
Rattus norvegicus | guanosine + phosphate | - |
? | |
5'-IMP + H2O | - |
Rattus norvegicus | inosine + phosphate | - |
? | |
5'-UMP + H2O | - |
Rattus norvegicus | uridine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure | Rattus norvegicus |
tetramer | 4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
enzyme from glioblastoma cells, stable up to | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.1 | enzyme from glioblastoma cells | Rattus norvegicus |