Literature summary for 3.1.3.5 extracted from

Turnay, J.; Olmo, N.; Navarro, J.M.; Gavilanes, J.G.; Lizarbe, M.A.
Isolation and characterization of the ecto-5'-nucleotidase from a rat glioblastoma cell line (1992), Mol. Cell. Biol., 117, 23-33.

Search for more literature for 3.1.3.5

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Rattus norvegicus
agglutinin	enzyme from glioblastoma cells	Rattus norvegicus
ATP	-	Rattus norvegicus
Co2+	strong, enzyme from glioblastoma cells	Rattus norvegicus
concanavalin A	enzyme from glioblastoma cells	Rattus norvegicus
EDTA	-	Rattus norvegicus
Mn2+	strong, enzyme from glioblastoma cells	Rattus norvegicus
Ni2+	strong, enzyme from glioblastoma cells	Rattus norvegicus
Zn2+	strong, enzyme from glioblastoma cells	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	5'-IMP	enzyme from glioblastoma cells	Rattus norvegicus
0.02	-	5'-AMP	enzyme from glioblastoma cells	Rattus norvegicus
0.031	-	5'-UMP	enzyme from glioblastoma cells	Rattus norvegicus
0.038	-	5'-GMP	enzyme from glioblastoma cells	Rattus norvegicus
0.07	-	5'-CMP	enzyme from glioblastoma cells	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Rattus norvegicus	5886	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
74000	-	2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure	Rattus norvegicus
74000	-	4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure	Rattus norvegicus
135000	-	enzyme from glioblastoma cells, gel filtration in presence of 0.6% sodium deoxycholate	Rattus norvegicus
268000	-	enzyme from glioblastoma cells, gel filtration in presence of 0.1% Triton X-100	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
glioblastoma cells, membrane-bound	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
glioblastoma cell	Rugli cells	Rattus norvegicus	-

Storage Stability

Storage Stability	Organism
-20°C, enzyme from glioblastoma cells, 40 mM Tris-HCl buffer, pH 7.4, 0.1 M NaCl, 0.1% Triton X-100, several months stable	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-AMP + H2O	-	Rattus norvegicus	adenosine + phosphate	-	?
5'-CMP + H2O	-	Rattus norvegicus	cytidine + phosphate	-	?
5'-GMP + H2O	-	Rattus norvegicus	guanosine + phosphate	-	?
5'-IMP + H2O	-	Rattus norvegicus	inosine + phosphate	-	?
5'-UMP + H2O	-	Rattus norvegicus	uridine + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure	Rattus norvegicus
tetramer	4 * 74000, enzyme from glioblastoma cells may have dimeric or tetrameric structure	Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
46	-	enzyme from glioblastoma cells, stable up to	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	8.1	enzyme from glioblastoma cells	Rattus norvegicus

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Release 2023.1 (January 2023)