Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.27 | - |
5-phosphoribosyl-1-diphosphate | at pH 8.9 and 25°C | Bacillus subtilis | |
1.53 | - |
5'-XMP | at pH 8.9 and 25°C | Bacillus subtilis | |
1.64 | - |
4-nitrophenyl phosphate | at pH 8.9 and 25°C | Bacillus subtilis | |
6.65 | - |
5'-GMP | at pH 8.9 and 25°C | Bacillus subtilis | |
24 | - |
D-ribose 5-phosphate | at pH 8.9 and 25°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for Mg2+, 5 mM is the optimal concentration | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 5'-ribonucleotide + H2O | Bacillus subtilis | - |
a ribonucleoside + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O32125 | - |
- |
Bacillus subtilis 168 | O32125 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Sephadex G-25 gel filtration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0245 | - |
at pH 8.9 and 25°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | best substrate, very high activity | Bacillus subtilis | 4-nitrophenol + phosphate | - |
? | |
5'-ADP + H2O | very low activity | Bacillus subtilis | AMP + phosphate | - |
? | |
5'-AMP + H2O | low activity | Bacillus subtilis | adenosine + phosphate | - |
? | |
5'-AMP + H2O | low activity | Bacillus subtilis 168 | adenosine + phosphate | - |
? | |
5'-ATP + H2O | very low activity | Bacillus subtilis | ADP + phosphate | - |
? | |
5'-CTP + H2O | low activity | Bacillus subtilis | CDP + phosphate | - |
? | |
5'-dAMP + H2O | low activity | Bacillus subtilis | deoxyadenosine + phosphate | - |
? | |
5'-dAMP + H2O | low activity | Bacillus subtilis 168 | deoxyadenosine + phosphate | - |
? | |
5'-dGMP + H2O | low activity | Bacillus subtilis | deoxyguanosine + phosphate | - |
? | |
5'-dIMP + H2O | low activity | Bacillus subtilis | deoxyinosine + phosphate | - |
? | |
5'-GDP + H2O | very low activity | Bacillus subtilis | GMP + phosphate | - |
? | |
5'-GMP + H2O | low activity | Bacillus subtilis | guanosine + phosphate | - |
? | |
5'-GTP + H2O | very low activity | Bacillus subtilis | GDP + phosphate | - |
? | |
5'-IDP + H2O | very low activity | Bacillus subtilis | IMP + phosphate | - |
? | |
5'-IMP + H2O | low activity | Bacillus subtilis | inosine + phosphate | - |
? | |
5'-UMP + H2O | low activity | Bacillus subtilis | uridine + phosphate | - |
? | |
5'-UMP + H2O | low activity | Bacillus subtilis 168 | uridine + phosphate | - |
? | |
5'-XMP + H2O | third best substrate | Bacillus subtilis | xanthosine + phosphate | - |
? | |
5-phosphoribosyl-1-diphosphate + H2O | - |
Bacillus subtilis | ? | - |
? | |
a 5'-ribonucleotide + H2O | - |
Bacillus subtilis | a ribonucleoside + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | very low activity | Bacillus subtilis | D-glucose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | second best substrate | Bacillus subtilis | D-ribose + phosphate | - |
? | |
D-ribose 5-phosphate + H2O | second best substrate | Bacillus subtilis 168 | D-ribose + phosphate | - |
? | |
ITP + H2O | very low activity | Bacillus subtilis | IDP + phosphate | - |
? | |
additional information | no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose | Bacillus subtilis | ? | - |
? | |
additional information | no activity with 3'-CMP, 3'-AMP, and UDP-D-glucose | Bacillus subtilis 168 | ? | - |
? | |
UTP + H2O | very low activity | Bacillus subtilis | UDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 29200, His6-tagged enzyme, calculated from amino acid sequence | Bacillus subtilis |
homodimer | 2 * 30000, His6-tagged enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
YutF | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
5'-GMP | at pH 8.9 and 25°C | Bacillus subtilis | |
0.17 | - |
5-phosphoribosyl-1-diphosphate | at pH 8.9 and 25°C | Bacillus subtilis | |
0.31 | - |
5'-XMP | at pH 8.9 and 25°C | Bacillus subtilis | |
1.84 | - |
D-ribose 5-phosphate | at pH 8.9 and 25°C | Bacillus subtilis | |
61 | - |
4-nitrophenyl phosphate | at pH 8.9 and 25°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | in 100 mM MES buffer with 5'-IMP as a substrate | Bacillus subtilis |
8.7 | 9 | in 100 mM Tris-HCl with 4-nitrophenyl phosphate as substrate | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
5'-GMP | at pH 8.9 and 25°C | Bacillus subtilis | |
0.077 | - |
D-ribose 5-phosphate | at pH 8.9 and 25°C | Bacillus subtilis | |
0.13 | - |
5-phosphoribosyl-1-diphosphate | at pH 8.9 and 25°C | Bacillus subtilis | |
0.21 | - |
5'-XMP | at pH 8.9 and 25°C | Bacillus subtilis | |
37 | - |
4-nitrophenyl phosphate | at pH 8.9 and 25°C | Bacillus subtilis |