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Literature summary for 3.1.3.5 extracted from

  • De Jesus, J.B.; Cosentino-Gomes, D.; Meyer-Fernandes, J.R.
    Characterization of an ecto-5'-nucleotidase activity present on the cell surface of Tritrichomonas foetus (2011), Vet. Parasitol., 179, 50-56.
    View publication on PubMed
Search for more literature for 3.1.3.5

Inhibitors

Inhibitors Comment Organism Structure
Ammonium molybdate
-
 Tritrichomonas suis
additional information no inhibition of the enzyme by phosphatase inhibitors. i.e. sodium orthovanadate and sodium fluoride Tritrichomonas suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Tritrichomonas suis
0.49
-
 5'-AMP pH 7.2, 36°C Tritrichomonas suis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface plasma membrane-bound ecto-enzyme activity Tritrichomonas suis 9986
-
extracellular plasma membrane-bound ecto-enzyme activity Tritrichomonas suis
-
-
plasma membrane plasma membrane-bound ecto-enzyme activity Tritrichomonas suis 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Mn2+, Sr2+, or Ca2+, as well as the metal chelators EGTA or EDTA, have no effect on the ecto-5'-nucleotidase activity Tritrichomonas suis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-AMP + H2O Tritrichomonas suis
-
 adenosine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tritrichomonas suis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Tritrichomonas suis adenosine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
CD73
-
 Tritrichomonas suis
ecto-5'-nucleotidase
-
 Tritrichomonas suis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
 assay at Tritrichomonas suis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Tritrichomonas suis

General Information

General Information Comment Organism
metabolism Trichomonas foetus is devoid of the ability to synthesize purine nucleotides de novo, depending instead on salvaging purines from the host environment. Ecto-5'-nucleotidase catalyzes the final step of extracellular nucleotide degradation, the hydrolysis of nucleoside 5'-monophosphates to the corresponding nucleosides and phosphate Tritrichomonas suis