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Literature summary for 3.1.3.5 extracted from

  • Pacheco-Aguilar, R.; Ramirez-Suarez, J.C.; Castillo-Yanez, F.J.; Pena-Ramos, E.A.; Valenzuela-Soto, E.M.; Marquez-Rios, E.
    Isolation and properties of 5'-nucleotidase isolated from jumbo squid (Dosidicus gigas) mantle muscle from the Gulf of California, Mexico (2008), Food Chem., 112, 880-884.
No PubMed abstract available
Search for more literature for 3.1.3.5

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.49
-
 AMP in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C Dosidicus gigas
6.34
-
 IMP in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C Dosidicus gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 3 * 33000, SDS-PAGE Dosidicus gigas
107000
-
 gel filtration Dosidicus gigas

Organism

Organism UniProt Comment Textmining
Dosidicus gigas
-
 jumbo squid
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, concanavalin A Sepharose column chromatography, and 5'-AMP Sepharose column chromatography Dosidicus gigas

Source Tissue

Source Tissue Comment Organism Textmining
muscle mantle muscle Dosidicus gigas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0054
-
 crude extract, in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C Dosidicus gigas
5.15
-
 after 953.7fold purification, in 40 mM sodium citrate buffer, 0.1 mM dithiothreitol, 110 mM NaCl, 20 mM MgCl2, and 20 mM CaCl2, at 50°C Dosidicus gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O
-
 Dosidicus gigas adenosine + phosphate
-
 ?
5'-IMP + H2O
-
 Dosidicus gigas inosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 33000, SDS-PAGE Dosidicus gigas

pI Value

Organism Comment pI Value Maximum pI Value
Dosidicus gigas isoelectric focusing, the isoelectric point of 5'-nucleotidase shows two protein bands that correspond to isoelectric points of 3.6 and 3.8
-
 3.6
Dosidicus gigas isoelectric focusing, the isoelectric point of 5'-nucleotidase shows two protein bands that correspond to isoelectric points of 3.6 and 3.8
-
 3.8