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Literature summary for 3.1.3.5 extracted from

  • Borges, F.P.; Gottardi, B.; Stuepp, C.; Larre, A.B.; Tasca, T.; De Carli, G.A.; Bonan, C.D.
    Characterization of an ecto-5'-nucleotidase (EC 3.1.3.5) activity in intact trophozoites of Trichomonas gallinae (2007), Vet. Parasitol., 143, 106-111.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ammonium molybdate almost complete inhibition at 1.0 mM Trichomonas gallinae
additional information insensitive to levamisole, tetramisole and adenosine 5'-[alpha-beta-methylene]diphosphate Trichomonas gallinae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.466
-
AMP in 50 mM Tris buffer (pH 7.2) and 3.0 mM MgCl2, at 37°C Trichomonas gallinae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Trichomonas gallinae

Organism

Organism UniProt Comment Textmining
Trichomonas gallinae
-
-
-
Trichomonas gallinae TG7
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Trichomonas gallinae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AMP + H2O 100% activity Trichomonas gallinae adenosine + phosphate
-
?
5'-AMP + H2O 100% activity Trichomonas gallinae TG7 adenosine + phosphate
-
?
5'-CMP + H2O 87% activity compared to 5'-AMP Trichomonas gallinae cytidine + phosphate
-
?
5'-CMP + H2O 87% activity compared to 5'-AMP Trichomonas gallinae TG7 cytidine + phosphate
-
?
5'-GMP + H2O 68% activity compared to 5'-AMP Trichomonas gallinae guanosine + phosphate
-
?
5'-GMP + H2O 68% activity compared to 5'-AMP Trichomonas gallinae TG7 guanosine + phosphate
-
?
5'-UMP + H2O 60% activity compared to 5'-AMP Trichomonas gallinae uridine + phosphate
-
?
5'-UMP + H2O 60% activity compared to 5'-AMP Trichomonas gallinae TG7 uridine + phosphate
-
?
diphosphate + H2O 5.8% activity compared to 5'-AMP Trichomonas gallinae 2 phosphate
-
?
diphosphate + H2O 5.8% activity compared to 5'-AMP Trichomonas gallinae TG7 2 phosphate
-
?

Synonyms

Synonyms Comment Organism
ecto-5'-nucleotidase
-
Trichomonas gallinae