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Literature summary for 3.1.3.5 extracted from

  • Kuznetsova, E.; Proudfoot, M.; Sanders, S.A.; Reinking, J.; Savchenko, A.; Arrowsmith, C.H.; Edwards, A.M.; Yakunin, A.F.
    Enzyme genomics: Application of general enzymatic screens to discover new enzymes (2005), FEMS Microbiol. Rev., 29, 263-279.
    View publication on PubMed
Search for more literature for 3.1.3.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa

General Stability

General Stability Organism
stable Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 5'-UMP +/- 0.02 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I767
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.14
-
 +/- 0.05, 5'-UMP Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-IMP + H2O
-
 Pseudomonas aeruginosa inosine + phosphate
-
 ?
5'-UMP + H2O
-
 Pseudomonas aeruginosa uridine + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Pseudomonas aeruginosa