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Literature summary for 3.1.3.5 extracted from

  • Hunsucker, S.A.; Spychala, J.; Mitchell, B.S.
    Human cytosolic 5'-nucleotidase I: characterization and role in nucleoside analog resistance (2001), J. Biol. Chem., 276, 10498-10504.
    View publication on PubMed
Search for more literature for 3.1.3.5

Activating Compound

Activating Compound Comment Organism Structure
ADP required for full activity, of the recombinant human enzyme, allosteric activator Homo sapiens
ATP activates Homo sapiens
GTP serves as activitor, not as strong as ADP Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-fluoroadenine-9-beta-D-arabinofuranoside 1 mM, 70% inhibition with 5 mM 5'-AMP as substrate Homo sapiens
araCMP 1 mM, 25% inhibition with 5 mM 5'-AMP as substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 dCMP pH 7.0, 37°C, in presence of 1 mM ADP Homo sapiens
0.193
-
 dCMP pH 7.0, 37°C, without ADP Homo sapiens
1.87
-
 5'-AMP pH 7.0, 37°C, in presence of 1 mM ADP Homo sapiens
2.47
-
 5'-IMP pH 7.0, 37°C, in presence of 1 mM ADP Homo sapiens
2.96
-
 5'-AMP pH 7.0, 37°C, without ADP Homo sapiens
12.11
-
 5'-IMP pH 7.0, 37°C, without ADP Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47700
-
 x * 47700, calculation from nucleotide sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BXI3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
heart high activity Homo sapiens
-
HEK-293 cell
-
 Homo sapiens
-
JURKAT cell
-
 Homo sapiens
-
kidney low activity Homo sapiens
-
pancreas
-
 Homo sapiens
-
skeletal muscle high activity Homo sapiens
-
testis low activity Homo sapiens
-
uterus low activity Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.4
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-2'-deoxyadenosine monophosphate + H2O 39% of the activity with 5'-AMP Homo sapiens 2-chloro-2'-deoxyadenosine + phosphate
-
 ?
5'-AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
5'-CMP + H2O 85% of the activity with 5'-AMP Homo sapiens cytidine + phosphate
-
 ?
5'-dIMP + H2O 61% of the activity with 5'-AMP Homo sapiens deoxyinosine + phosphate
-
 ?
5'-GMP + H2O 57% of the activity with 5'-AMP Homo sapiens guanosine + phosphate
-
 ?
5'-IMP + H2O
-
 Homo sapiens inosine + phosphate
-
 ?
5'-TMP + H2O 13% of the activity with 5'-AMP Homo sapiens thymidine + phosphate
-
 ?
5'-UMP + H2O 78% of the activity with 5'-AMP Homo sapiens uridine + phosphate
-
 ?
dAMP + H2O 91% of the activity with 5'-AMP Homo sapiens deoxyadenosine + phosphate
-
 ?
dCMP + H2O 10% of the activity with 5'-AMP Homo sapiens deoxycytidine + phosphate
-
 ?
dGMP + H2O 75% of the activity with 5'-AMP Homo sapiens deoxyguanosine + phosphate
-
 ?
dIMP + H2O 84% of the activity with 5'-AMP Homo sapiens deoxyinosine + phosphate
-
 ?
dUMP + H2O 7% of the activity with 5'-AMP Homo sapiens deoxyuridine + phosphate
-
 ?
additional information no dephosphorylation of araCMP and 2-fluoroadenine-9-beta-D-arabinofuranoside Homo sapiens ?
-
 ?
additional information cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficiacy of certain nucleoside analogs Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47700, calculation from nucleotide sequence Homo sapiens

Synonyms

Synonyms Comment Organism
5'-nucleotidase I
-
 Homo sapiens