Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | activates | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene PTPRE, located on chromosome 12p12-p13, recombinant expression of isolated C-terminally His6-tagged intracellular catalytic domain of PTP-oc (DELTAPTP-oc) in Escherichia coli strain BL21(DE3), method evaluation | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of the isolated intracellular catalytic domain of PTP-oc (DELTAPTP-oc) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | inhibits the catalytic domain by 60% at 2 M | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.8018 | - |
4-nitrophenyl phosphate | pH 7.0, 37°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a structurally unique transmembrane protein tyrosine phosphatase | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Homo sapiens | - |
[a protein]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23469 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant isolated His6-tagged intracellular catalytic domain of PTP-oc 6.8fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte | - |
Homo sapiens | - |
additional information | osteoclastic protein tyrosine phosphatase (PTP-oc) is mainly expressed in precursors of osteoclasts, including B lymphocytes, cells of the monocyte-macrophage lineage, and mature osteoclasts | Homo sapiens | - |
osteoclast | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15700 | - |
purified recombinant catalytic domain, pH 7.0, 37°C, substrate 4-nitrophenyl phosphate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Homo sapiens | [a protein]-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | osteoclastic protein tyrosine phosphatase (PTP-oc) is a structurally unique transmembrane protein tyrosine phosphatase (PTP) that contains only a relatively small intracellular PTP catalytic domain, does not have an extracellular domain, and lacks a signal peptide proximal to the N-terminus | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
osteoclastic protein-tyrosine phosphatase | - |
Homo sapiens |
PTP | - |
Homo sapiens |
PTP-oc | - |
Homo sapiens |
PTPRE | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant intracellular catalytic domain of PTP-oc (DELTAPTP-oc) | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | 42 | over 50% of maximal activity within this range, recombinant catalytic enzyme domain, profile overview | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant intracellular catalytic domain of PTP-oc (DELTAPTP-oc) | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | activity range of the recombinant catalytic enzyme domain, profile overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | osteoclastic protein tyrosine phosphatase (PTP-oc) belongs to the receptor PTP family | Homo sapiens |
additional information | osteoclastic protein tyrosine phosphatase (PTP-oc) is a structurally unique transmembrane protein tyrosine phosphatase (PTP) that contains only a relatively small intracellular PTP catalytic domain, does not have an extracellular domain, and lacks a signal peptide proximal to the N-terminus | Homo sapiens |
physiological function | osteoclastic protein tyrosine phosphatase (PTP-oc) is an activator of osteoclast differentiation and activity | Homo sapiens |