Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.48 extracted from

  • Ahmad, Z.; Morona, R.; Standish, A.J.
    In vitro characterization and identification of potential substrates of a low molecular weight protein tyrosine phosphatase in Streptococcus pneumoniae (2018), Microbiology, 164, 697-703 .
    View publication on PubMed
Search for more literature for 3.1.3.48

Cloned(Commentary)

Cloned (Comment) Organism
gene SPD_1837, recombinant expression in Escherichia coli strain BL21(DE3) Streptococcus pneumoniae D39

Protein Variants

Protein Variants Comment Organism
C8S site-directed mutagenesis, mutation in the putative active site cysteine, almost inactive enzyme mutant Streptococcus pneumoniae D39

Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid
-
 Streptococcus pneumoniae D39
sodium orthovanadate
-
 Streptococcus pneumoniae D39

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Streptococcus pneumoniae D39
8
-
 4-nitrophenyl phosphate recombinant enzyme, pH 7.0, 37°C Streptococcus pneumoniae D39

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15800
-
 recombinant enzyme, gel filtration Streptococcus pneumoniae D39

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[a protein]-tyrosine phosphate + H2O Streptococcus pneumoniae D39
-
 [a protein]-tyrosine + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O Streptococcus pneumoniae D39 NCTC 7466
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae D39 A0A0H2ZQ64
-
-
Streptococcus pneumoniae D39 NCTC 7466 A0A0H2ZQ64
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Streptococcus pneumoniae D39 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Streptococcus pneumoniae D39 NCTC 7466 4-nitrophenol + phosphate
-
 ?
DADE(pY)LIPQQG + H2O phosphopeptide-2 Streptococcus pneumoniae D39 DADEYLIPQQG + phosphate
-
 ?
DADE(pY)LIPQQG + H2O phosphopeptide-2 Streptococcus pneumoniae D39 NCTC 7466 DADEYLIPQQG + phosphate
-
 ?
END(pY)INASL + H2O phosphopeptide-1 Streptococcus pneumoniae D39 ENDYINASL + phosphate
-
 ?
END(pY)INASL + H2O phosphopeptide-1 Streptococcus pneumoniae D39 NCTC 7466 ENDYINASL + phosphate
-
 ?
additional information Spd1837 has activity against two phosphotyrosine-containing peptides, phosphopeptide-1 (END(pY)INASL) and phosphopeptide-2 (DADE(pY)LIPQQG), as the mutant protein Spd1837C8S lacks activity against these two phosphotyrosine-containing phosphopeptides Streptococcus pneumoniae D39 ?
-
 ?
additional information Spd1837 has activity against two phosphotyrosine-containing peptides, phosphopeptide-1 (END(pY)INASL) and phosphopeptide-2 (DADE(pY)LIPQQG), as the mutant protein Spd1837C8S lacks activity against these two phosphotyrosine-containing phosphopeptides Streptococcus pneumoniae D39 NCTC 7466 ?
-
 ?
[a protein]-tyrosine phosphate + H2O
-
 Streptococcus pneumoniae D39 [a protein]-tyrosine + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O
-
 Streptococcus pneumoniae D39 NCTC 7466 [a protein]-tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 15800, recombinant enzyme, SDS-PAGE Streptococcus pneumoniae D39

Synonyms

Synonyms Comment Organism
LMWPTP
-
 Streptococcus pneumoniae D39
low molecular weight protein tyrosine phosphatase
-
 Streptococcus pneumoniae D39
protein tyrosine phosphatase
-
 Streptococcus pneumoniae D39
PTP
-
 Streptococcus pneumoniae D39
Spd1837
-
 Streptococcus pneumoniae D39
SPD_1837
-
 Streptococcus pneumoniae D39

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptococcus pneumoniae D39

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 activity range, profile overview Streptococcus pneumoniae D39

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Streptococcus pneumoniae D39

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 activity range, profile overview Streptococcus pneumoniae D39