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Literature summary for 3.1.3.48 extracted from
- The protein-tyrosine phosphatase DEP-1 promotes migration and phagocytic activity of microglial cells in part through negative regulation of fyn tyrosine kinase (2017), Glia, 65, 416-428 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Ptprj, quantitative PCR expression analysis | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of specific shRNA cell lines for DEP-1 knockout | Mus musculus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Mus musculus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | in vivo phagocytosis assay and in vivo microglial migration assay, overview | ? | - |
? | |
| additional information | Mus musculus C57BL/6 | in vivo phagocytosis assay and in vivo microglial migration assay, overview | ? | - |
? | |
| [a protein]-tyrosine phosphate + H2O | Mus musculus | - |
[a protein]-tyrosine + phosphate | - |
? | |
| [a protein]-tyrosine phosphate + H2O | Mus musculus C57BL/6 | - |
[a protein]-tyrosine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q64455 | - |
- |
| Mus musculus C57BL/6 | Q64455 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| BV-2 cell | - |
Mus musculus | - |
| cerebral cortex | - |
Mus musculus | - |
| microglia | murine microglia are isolated from newborn mice, DEP-1 is expressed in microglial cells in a cell density-dependent manner | Mus musculus | - |
| additional information | DEP-1 protein levels increase in several cell types at high-cell density. DEP-1 is a widely expressed in cells of the immune system | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | in vivo phagocytosis assay and in vivo microglial migration assay, overview | Mus musculus | ? | - |
? | |
| additional information | in vivo phagocytosis assay and in vivo microglial migration assay, overview | Mus musculus C57BL/6 | ? | - |
? | |
| [a protein]-tyrosine phosphate + H2O | - |
Mus musculus | [a protein]-tyrosine + phosphate | - |
? | |
| [a protein]-tyrosine phosphate + H2O | - |
Mus musculus C57BL/6 | [a protein]-tyrosine + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CD148 | - |
Mus musculus |
| density-enhanced phosphatase-1 | - |
Mus musculus |
| DEP-1 | - |
Mus musculus |
| DEP-1 phosphatase | - |
Mus musculus |
| protein-tyrosine phosphatase | - |
Mus musculus |
| PTP | - |
Mus musculus |
| PTPRJ | - |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | in the microglial cell line BV-2, DEP-1 depletion by shRNA-mediated knockdown results in enhanced phosphorylation of the Fyn activating tyrosine (Tyr420) and elevated specific Fyn-kinase activity in immunoprecipitates. Fyn mRNA and protein levels are reduced in DEP-1-deficient microglia cells. Knockdown of DEP-1 causes a small but significant decrease of phagocytosis of unstimulated microglial BV-2 cells. And the stimulatory effect on phagocytosis is significantly reduced in cells depleted for DEP-1, reduced phagocytic activity of microglial cells in DEP-1-/- mice brains | Mus musculus |
| physiological function | density-enhanced phosphatase-1 (DEP-1), also designated PTPRJ, receptor-type PTP eta or CD148, is a transmembrane PTP endowed with one intracellular catalytic domain, and an extracellular domain harboring eight fibronectin-like repeats. The protein-tyrosine phosphatase DEP-1 promotes migration and phagocytic activity of microglial cells in part through negative regulation of fyn tyrosine kinase. DEP-1 protein levels increase in several cell types at high-cell density, suggesting a role of the molecule in density-dependent growth control. A negative regulatory role of Fyn for microglial functions is inhibited by DEP-1. Determination of a role of DEP-1 in a potential DEP-1-Fyn axis in the regulation of microglial functions. Enzyme DEP-1 stimulates microglial phagocytosis and microglial cell migration, overview | Mus musculus |
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Release 2023.1 (January 2023)
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