Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.48 extracted from

  • Stehle, T.; Sreeramulu, S.; Loehr, F.; Richter, C.; Saxena, K.; Jonker, H.R.; Schwalbe, H.
    The apo-structure of the low molecular weight protein-tyrosine phosphatase A (MptpA) from Mycobacterium tuberculosis allows for better target-specific drug development (2012), J. Biol. Chem., 287, 34569-34582.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 x * 17500, calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[a protein]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIA1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation at Tyr128 and Tyr129 is catalyzed by the kinase PtkA Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis 4-nitrophenol + phosphate
-
 ?
[a protein]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [a protein]-tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 17500, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
LMW-PTP
-
 Mycobacterium tuberculosis
low molecular weight protein-tyrosine phosphatase A
-
 Mycobacterium tuberculosis
MPtpA
-
 Mycobacterium tuberculosis
protein-tyrosine phosphatase
-
 Mycobacterium tuberculosis
PTP
-
 Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function low molecular weight protein-tyrosine phosphatase A is a key virulence factor of Mycobacterium tuberculosis, the enzyme is essential for infection of host macrophages Mycobacterium tuberculosis