Cloned (Comment) | Organism |
---|---|
expression of the active and soluble PTPase in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | - |
Thermus thermophilus | |
Cu2+ | - |
Thermus thermophilus | |
SO42- | - |
Thermus thermophilus | |
Zn2+ | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.01 | - |
4-nitrophenyl phosphate | pH 3.8, 75°C, recombinant enzyme | Thermus thermophilus | |
37.88 | - |
4-nitrophenyl phosphate | pH 3.8, 30°C, recombinant enzyme | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates the recombinant enzyme | Thermus thermophilus | |
Ca2+ | activates the recombinant enzyme | Thermus thermophilus | |
Mg2+ | activates the recombinant enzyme | Thermus thermophilus | |
Mn2+ | activates 3fold at 5 mM, recombinant enzyme | Thermus thermophilus | |
Ni2+ | activates the recombinant enzyme | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation | Thermus thermophilus |
22260 | - |
x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72JF6 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72JF6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble PTPase 54.3fold from Escherichia coli strain BL21(DE3) by heat treatment and gel filtration to over 95% purity | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.02 | - |
purified recombinant enzyme | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Thermus thermophilus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, recombinant enzyme, SDS-PAGE, x * 22260, sequence calculation | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
low-molecular-weight protein tyrosine phosphatase | - |
Thermus thermophilus |
PTPase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
recombinant enzyme | Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | over 50% of maximal activity at 50-80°C, temperature profile, overview | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
4 h, purified recombinant enzyme, no significant decrease in activity | Thermus thermophilus |
60 | - |
4 h, purified recombinant enzyme, no significant decrease in activity | Thermus thermophilus |
70 | - |
4 h, purified recombinant enzyme, loss of 34% activity | Thermus thermophilus |
80 | - |
1 h, purified recombinant enzyme, 30% activity remaining | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
670.9 | - |
4-nitrophenyl phosphate | pH 3.8, 30°C, recombinant enzyme | Thermus thermophilus | |
4013 | - |
4-nitrophenyl phosphate | pH 3.8, 75°C, recombinant enzyme | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.8 | 4.8 | recombinant enzyme | Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.2 | 10 | pH profile, overview | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | the heat-resistant PTPase may play important roles in vivo in the adaptation of the microorganism to extreme temperatures and specific nutritional conditions | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.71 | - |
4-nitrophenyl phosphate | pH 3.8, 30°C, recombinant enzyme | Thermus thermophilus | |
667.8 | - |
4-nitrophenyl phosphate | pH 3.8, 75°C, recombinant enzyme | Thermus thermophilus |