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Literature summary for 3.1.3.48 extracted from

  • Li, Z.; Wang, Z.; Peng, G.; Yin, Y.; Zhao, H.; Cao, Y.; Xia, Y.
    Purification and characterization of a novel thermostable extracellular protein tyrosine phosphatase from Metarhizium anisopliae strain CQMa102 (2006), Biosci. Biotechnol. Biochem., 70, 1961-1968.
    View publication on PubMed
Search for more literature for 3.1.3.48

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 6% increase of activity at 20 mM Metarhizium anisopliae
dithiothreitol 7.4% increase of activity at 20 mM Metarhizium anisopliae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 16.6% inhibition at 2 mM Metarhizium anisopliae
Cu2+ 32.2% inhibition at 2 mM Metarhizium anisopliae
Fe2+ 34.5% inhibition at 2 mM Metarhizium anisopliae
molybdate 92.2% inhibition at 5 mM Metarhizium anisopliae
additional information EDTA, sodium tartrate, sodium fluoride, or okadaic acid have no effect on activity Metarhizium anisopliae
N-Methylmaleimide 76.3% inhibition at 0.5 mM Metarhizium anisopliae
orthovanadate 24.5% inhibition at 5 mM Metarhizium anisopliae
tungstate 16.3% inhibition at 20 mM Metarhizium anisopliae
Zn2+ 75.2% inhibition at 5 mM Metarhizium anisopliae

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 42.5% increase of activity at 20 mM Metarhizium anisopliae
Mn2+ 9.2% increase of activity at 20 mM Metarhizium anisopliae
additional information Mg2+, Co2+, or Ca2+ have no effect on activity Metarhizium anisopliae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82500
-
 SDS-PAGE Metarhizium anisopliae

Organism

Organism UniProt Comment Textmining
Metarhizium anisopliae
-
-
-
Metarhizium anisopliae CQMa102
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ConA-Sepharose 4B column chromatography, HighQ anion resin chromatography, and 25S cation resin column chromatography Metarhizium anisopliae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.045
-
 culture medium, using p-nitrophenyl phosphate as a substrate Metarhizium anisopliae
8.681
-
 after 192.9fold purification, using p-nitrophenyl phosphate as a substrate Metarhizium anisopliae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O 9.5% activity compared to TRDIpYETDYYRK Metarhizium anisopliae ADP + phosphate
-
 ?
ATP + H2O 9.5% activity compared to TRDIpYETDYYRK Metarhizium anisopliae CQMa102 ADP + phosphate
-
 ?
additional information no activity against phytic acid, ADP, AMP, O-phospho-L-serine or O-phospho-L-threonine Metarhizium anisopliae ?
-
 ?
additional information no activity against phytic acid, ADP, AMP, O-phospho-L-serine or O-phospho-L-threonine Metarhizium anisopliae CQMa102 ?
-
 ?
O-phospho-L-tyrosine + H2O
-
 Metarhizium anisopliae L-tyrosine + phosphate
-
 ?
O-phospho-L-tyrosine + H2O
-
 Metarhizium anisopliae CQMa102 L-tyrosine + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 60.3% activity compared to TRDIpYETDYYRK Metarhizium anisopliae p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 60.3% activity compared to TRDIpYETDYYRK Metarhizium anisopliae CQMa102 p-nitrophenol + phosphate
-
 ?
TRDIpYETDYYRK + H2O 100% activity Metarhizium anisopliae TRDIYETDYYRK + phosphate
-
 ?
TRDIpYETDYYRK + H2O 100% activity Metarhizium anisopliae CQMa102 TRDIYETDYYRK + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
protein tyrosine phosphatase
-
 Metarhizium anisopliae
PTPase
-
 Metarhizium anisopliae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Metarhizium anisopliae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 70 remains stable after 1 h at up to 70°C Metarhizium anisopliae
80
-
 loses 31% activity after 10 min at 80°C Metarhizium anisopliae
90
-
 complete loss of activity after 10 min at 90°C Metarhizium anisopliae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Metarhizium anisopliae

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9.5 at 30°C Metarhizium anisopliae
4.5 8 stable at 70°C, but activity decreases markedly at pH below 4.5 or pH above 8.0 Metarhizium anisopliae

pI Value

Organism Comment pI Value Maximum pI Value
Metarhizium anisopliae isoelectric focusing
-
 9.5