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Literature summary for 3.1.3.47 extracted from
- Purification and properties of rat liver hydroxymethylglutaryl CoA reductase phosphatases (1981), Biochim. Biophys. Acta, 663, 211-221.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fluoride | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000065 | - |
[hydroxymethylglutaryl-CoA reductase]-phosphate | for the cytosolic isoenzyme IIa | Rattus norvegicus | |
| 0.000013 | - |
[hydroxymethylglutaryl-CoA reductase]-phosphate | for the cytosolic isoenzyme Ia | Rattus norvegicus | |
| 0.0000195 | - |
[hydroxymethylglutaryl-CoA reductase]-phosphate | for the catalytic subunit of ca. 35000 Da | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 310000 | - |
cytosolic isoenzyme IIa, gel chromatography | Rattus norvegicus |
| 480000 | - |
cytosolic isoenzyme Ia, gel chromatography | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O | Rattus norvegicus | - |
? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | after treatment with this solvent a catalytic subunit of 32000-35000 Da is obtained, activity increased | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 310 | 315 | no comments on grade of purification, native cytosolic isoenzyme Ia | Rattus norvegicus |
| 670 | 710 | no comments on grade of purification, native cytosolic isoenzyme IIa | Rattus norvegicus |
| 21480 | - |
purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -40°C, several months, no measurable loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O | - |
Rattus norvegicus | [hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate | - |
? | |
| [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O | - |
Rattus norvegicus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | for cytosolic isoenzymes Ia and IIa | Rattus norvegicus |
| 6 | 6.5 | for the catalytic subunit of ~35000 Da retrieved after ethanol treatment | Rattus norvegicus |
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