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Literature summary for 3.1.3.46 extracted from

  • Pilkis, S.J.; Claus, T.H.; Kountz, P.D.; El-Maghrabi, M.R.
    Enzymes of the fructose 6-phosphate-fructose 1,6-bisphosphate substrate cycle (1987), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 18, 3-46.
No PubMed abstract available
Search for more literature for 3.1.3.46

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Rattus norvegicus
glycerol 1-phosphate activation at high substrate concentrations Rattus norvegicus
GTP
-
 Rattus norvegicus
phosphate activates at high substrate concentrations Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,5-anhydro-D-mannitol 6-phosphate
-
 Rattus norvegicus
D-psicose 6-phosphate poor inhibitor Rattus norvegicus
D-ribose 5-phosphate poor inhibitor Rattus norvegicus
D-tagatose 6-phosphate poor inhibitor Rattus norvegicus
diethyldicarbonate inactivation Rattus norvegicus
fructose 6-phosphate noncompetetive Bos taurus
fructose 6-phosphate noncompetetive Rattus norvegicus
glycerol 1-phosphate inhibition at low substrate concentrations Rattus norvegicus
L-Sorbose 6-phosphate
-
 Rattus norvegicus
p-mercuribenzoate
-
 Rattus norvegicus
phosphate
-
 Bos taurus
phosphate inhibition at low substrate concentrations Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus
0.02
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration or sucrose density gradient centrifugation Rattus norvegicus
100000
-
 gel filtration or sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fructose 2,6-bisphosphate + H2O Rattus norvegicus
-
 ?
-
 ?
fructose 2,6-bisphosphate + H2O Bos taurus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 overview
-
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Rattus norvegicus
a
beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20 40
-
 Rattus norvegicus
20 40
-
 Bos taurus

Storage Stability

Storage Stability Organism
-70°C for 3 months including 100 mM KCl, 0.5 mM dithiothreitol, 20% glycerol and 50 mM Tris-HCl at pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus fructose 6-phosphate + phosphate
-
 ?
fructose 2,6-bisphosphate + H2O
-
 Bos taurus fructose 6-phosphate + phosphate
-
 ?
fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
fructose 2,6-bisphosphate + H2O
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000-55000, SDS-PAGE Rattus norvegicus
dimer 2 * 50000-55000, SDS-PAGE Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus