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Literature summary for 3.1.3.43 extracted from

  • Roche, T.E.; Cate, R.L.
    Purification of porcine liver pyruvate dehydrogenase complex and characterization of its catalytic and regulatory properties (1977), Arch. Biochem. Biophys., 183, 664-677.
    View publication on PubMed

General Stability

General Stability Organism
rabbit serum stabilizes Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
EGTA with Mg2+ as cofactor Sus scrofa
NADH 0.5 mM with 10 mM Mg2+ and 0.1 mM Ca2+ Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Km: 0.001-0.003 mM Sus scrofa
Ca2+ overcomes EGTA inhibition Sus scrofa
KCl
-
Sus scrofa
Mg2+ Km: 4.2 mM Sus scrofa
Mn2+ Km: 1 mM Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Sus scrofa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
Sus scrofa [pyruvate dehydrogenase (lipoamide)] + phosphate
-
ir
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
Sus scrofa ?
-
?