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Literature summary for 3.1.3.43 extracted from

  • Pratt, M.L.; Maher, J.F.; Roche, T.E.
    Purification of bovine kidney and heart pyruvate dehydrogenase phosphatase on Sepharose derivatized with the pyruvate dehydrogenase complex (1982), Eur. J. Biochem., 125, 349-355.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Bos taurus
Mg2+
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
1 * 89000 + 1 * 49000, gel electrophoresis, heterodimer Bos taurus
89000
-
1 * 89000 + 1 * 49000, gel electrophoresis, heterodimer Bos taurus
140000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O Bos taurus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
assay method Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Bos taurus
-
kidney
-
Bos taurus
-

Storage Stability

Storage Stability Organism
-70°C, 400 mM KCl for 2 months no loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
Bos taurus [pyruvate dehydrogenase (lipoamide)] + phosphate
-
ir
[pyruvate dehydrogenase (lipoamide)]-phosphate + H2O
-
Bos taurus ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 89000 + 1 * 49000, gel electrophoresis, heterodimer Bos taurus