Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulates the 4-nitrophenyl phosphatase activity 3.5fold at concentrations of 0.001-0.030 mM, but inhibits it at concentrations above 0.5 mM, mechanism | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulates the 4-nitrophenyl phosphatase activity at concentrations of 0.001-0.030 mM, but inhibits it at concentrations above 0.5 mM, mechanism | Escherichia coli | |
Fluorescein isothiocyanate | i.e. FITC, inhibits both the ATPase and 4-nitrophenyl phosphatase activities of the enzyme, fluorescence labeling, inhibition depends on pH | Escherichia coli | |
o-vanadate | inhibits the 4-nitrophenyl phosphatase activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the 4-nitrophenyl phosphatase activity is dependent on Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | Escherichia coli | - |
4-nitrophenol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Escherichia coli | 4-nitrophenol + phosphate | - |
? | |
additional information | the Kdp-ATPase, i.e. KdpA, the potassium channel part of the KdpFABC complex, of Escherichia coli shows 4-nitrophenyl phosphatase activity | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
p-nitrophenyl phosphatase | - |
Escherichia coli |
pNPP | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, 4-nitrophenyl phosphatase activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at, 4-nitrophenyl phosphatase activity | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
o-vanadate | pH 7.8, 37°C, inhibition of 4-nitrophenyl phosphatase activity | Escherichia coli | |
0.5 | - |
ATP | about, pH 7.8, 37°C, inhibition of 4-nitrophenyl phosphatase activity | Escherichia coli |