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Literature summary for 3.1.3.4 extracted from

  • Kim, H.B.; Kumar, A.; Wang, L.; Liu, G.H.; Keller, S.R.; Lawrence, J.C.; Finck, B.N.; Harris, T.E.
    Lipin 1 represses NFATc4 transcriptional activity in adipocytes to inhibit secretion of inflammatory factors (2010), Mol. Cell. Biol., 30, 3126-3139.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
coding sequence of lipin 1b subcloned downstream of the GST tag in the pEBG expression vector, transfected into 293T cells. Adenoviral constructs for HA-tagged lipin 1b Mus musculus

Protein Variants

Protein Variants Comment Organism
D712E lacks enzymatic activity, does not affect the interaction of lipin 1 and PPARalpha, but eliminates the ability of lipin 1 to interact with nuclear factor of activated T-cells c4. Also fails to repress nuclear factor of activated T-cells c4 transcriptional activity Mus musculus
I706F lacks enzymatic activity, retains the ability to interact with and repress nuclear factor of activated T-cells c4 transcriptional activity Mus musculus
I707F lacks enzymatic activity, retains the ability to interact with and repress nuclear factor of activated T-cells c4 transcriptional activity Mus musculus
I723F lacks enzymatic activity, is unable to interact with nuclear factor of activated T-cells c4 and has no repressive effect on reporter gene activity Mus musculus
L724F lacks enzymatic activity, is unable to interact with nuclear factor of activated T-cells c4 and has no repressive effect on reporter gene activity Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ lipin 1-mediated repression of nuclear factor of activated T-cells transcriptional activity occurs after nuclear factor of activated T-cells mobilization and activation by calcium Mus musculus
Mg2+ dependent on Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
3T3-L1 cell
-
 Mus musculus
-
adipocyte
-
 Mus musculus
-
preadipocyte
-
 Mus musculus
-

Synonyms

Synonyms Comment Organism
lipin 1
-
 Mus musculus
Mg2+-dependent phosphatidic acid phosphatase
-
 Mus musculus
PAP
-
 Mus musculus

Expression

Organism Comment Expression
Mus musculus lipin 1 knockdown by shRNA, decline in lipin 1 expression with increasing obesity in ob/ob mice down

General Information

General Information Comment Organism
malfunction in fat pads from mice deficient for lipin 1 (fld mice) and in 3T3-L1 adipocytes depleted of lipin 1 there is increased expression of several nuclear factor of activated T-cells target genes including TNFalpha, resistin, FABP4 and PPARgamma. Lipin 1 with the highly conserved amino-terminal NLIP domain deleted (DELTAN) is capable of both interaction and repression Mus musculus
physiological function lipin 1 is a potentially important link between triacylglycerol synthesis and adipose tissue inflammation. Lipin 1 represses nuclear factor of activated T-cells c4 transcriptional activity through protein-protein interaction and in the context of at least two different composite elements. Specific residues required for interaction with lipin 1 are contained within the RHD/DNA binding domain and carboxy terminus of nuclear factor of activated T-cells c4. Lipin 1 is present at the promoters of nuclear factor of activated T-cells c4 transcriptional targets in vivo. Lipin 1 protein and total PAP activity are decreased with increasing adiposity in the visceral, but not subcutaneous, fat pads of ob/ob mice. Lipin 1 can act to repress or activate transcription factors. Lipin 1 interacts with nuclear factor of activated T-cells c4 bound to DNA and is present at the promoters of nuclear factor of activated T-cells target genes. Lipin 1 recruits a histone deacetylase to repress nuclear factor of activated T-cells c4 transcription Mus musculus