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Literature summary for 3.1.3.4 extracted from

  • Han, G.S.; Carman, G.M.
    Characterization of the human LPIN1-encoded phosphatidate phosphatase isoforms (2010), J. Biol. Chem., 285, 14628-14638.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.4

Cloned(Commentary)

Cloned (Comment) Organism
full-length triple HA-tagged lipin 2 cloned into pCDNA3 expression construct, subcloned into the Ad-track cytomegalovirus vector, and recombined into the Ad-EASY system. Full-length cDNA for lipin 3 from pSPORT-lipin 3 expression vector Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
shRNA lipin 2 shRNA treatment significantly reduces hepatocyte PAP-1 activity in both wild-type and fld hepatocytes Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm majority of lipin 2 Mus musculus 5737
-
endoplasmic reticulum membrane lipin 2 Mus musculus 5789
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lipid droplet lipin 2 Mus musculus 5811
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nucleus lipin 2 Mus musculus 5634
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Organism

Organism UniProt Comment Textmining
Mus musculus
-
 male C57BL/6 mice
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Source Tissue

Source Tissue Comment Organism Textmining
hepatocyte lipin 2 Mus musculus
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liver lipin 2 is enriched in fatty liver dystrophic (fld) mice, whereas lipin 1 and lipin 3 protein is absent Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidic acid + H2O
-
 Mus musculus 1,2-diacyl-sn-glycerol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
lipin 1
-
 Mus musculus
lipin 2
-
 Mus musculus
lipin 3
-
 Mus musculus
PAP-1
-
 Mus musculus
phosphatidic acid phosphohydrolase
-
 Mus musculus

Expression

Organism Comment Expression
Mus musculus RNAi against lipin 2 markedly reduces PAP-1 activity in hepatocytes from both wild-type and fld mice and suppresses triglyceride synthesis under conditions of high fatty acid availability down
Mus musculus lipin 2 protein content is increased in fld liver independent of changes in steady-state Lpin2 mRNA levels. Lipin 2 is dynamically regulated in liver but is not a target gene of peroxisome proliferator-activated receptorgamma-coactivator 1alpha additional information
Mus musculus both lipin 2 mRNA and protein expression are significantly induced by food deprivation. Lipin 2 knockdown leads to a compensatory increase in Lpin3 mRNA expression, especially in fld hepatocytes up

General Information

General Information Comment Organism
malfunction lipin 1 deficiency does not affect PAP-1 activity in neonatal mice and leads to hepatic triglyceride accumulation. Loss of lipin 2 markedly impairs hepatocyte PAP-1 activity but does not affect basal rates of triglyceride synthesis. Lipin 2 knockdown abrogates triglyceride synthesis under conditions of increased fatty acid availability Mus musculus
physiological function lipin 2 plays an important role as a hepatic PAP-1 enzyme. Lipin 2 overexpression increases PAP-1 activity in HepG2 cells. Increased hepatic expression of lipin 2 plays a role in increased hepatic triglyceride synthesis rates in ob/ob mice Mus musculus