Literature summary for 3.1.3.4 extracted from

Gropler, M.C.; Harris, T.E.; Hall, A.M.; Wolins, N.E.; Gross, R.W.; Han, X.; Chen, Z.; Finck, B.N.
Lipin 2 is a liver-enriched phosphatidate phosphohydrolase enzyme that is dynamically regulated by fasting and obesity in mice (2009), J. Biol. Chem., 284, 6763-6772.

Search for more literature for 3.1.3.4

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency, food deprivation, and obesity, often independent of changes in steady-state mRNA levels. Fasting induces expression of Lpin1 and Lpin2 in wild-type and PGC-1alpha-deficient mice	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
expression analysis of lipin-1 and lipin-2, recombinant expression of lipin-2 in Hep-G2 cells using an adenoviral vector	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	livers of fld mice lacking lipin 1 exhibit normal PAP-1 activity and a 20fold increase in triglyceride levels, while the hepatic content of phosphatidate is markedly diminished in fld mice. Other phospholipids derived from phosphatidate, phosphatidylglycerol and cardiolipin, are also depleted. Hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency via a posttranscriptional mechanism	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes	Mus musculus	5829	-
endoplasmic reticulum membrane	lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes	Mus musculus	5789	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a 3-sn-phosphatidate + H2O	Mus musculus	lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme	a 1,2-diacyl-sn-glycerol + phosphate	-	?
additional information	Mus musculus	lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	genes Lpin1 and Lpin2	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	serine 106 residue of lipin-1 and lipin-2 is a major site of phosphorylation	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	lipin-1 and high activity of lipin-2	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a 3-sn-phosphatidate + H2O	-	Mus musculus	a 1,2-diacyl-sn-glycerol + phosphate	-	?
a 3-sn-phosphatidate + H2O	lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme	Mus musculus	a 1,2-diacyl-sn-glycerol + phosphate	-	?
additional information	lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
lipin-1	-	Mus musculus
lipin-2	-	Mus musculus
More	the enzyme is a member of the lipin family	Mus musculus
PAP-1	-	Mus musculus
phosphatidate phosphohydrolase	-	Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Mus musculus

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Release 2023.1 (January 2023)