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Literature summary for 3.1.3.4 extracted from

  • Sigal, Y.J.; Quintero, O.A.; Cheney, R.E.; Morris, A.J.
    Cdc42 and ARP2/3-independent regulation of filopodia by an integral membrane lipid-phosphatase-related protein (2007), J. Cell Sci., 120, 340-352.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information regulation of filopodia by LPR1 is not mediated by cdc42 or Rif, and is independent of the Arp2/3 complex Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
LPP1 and LPR1 expressed in Sf9 cells using a baculovirus vector. Recombinant LPR1 and mutants incorporating a C-terminal EGFP tag expressed in HeLa and COS7 cells. Overexpression of EGFP-LPR1 in SK-OV-3 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information LPR1 variants containing non-conservative substitutions of Ser198 and His200 within the C2 motif and Arg246 from the C3 motif are strongly expressed in transient transfection experiments but fail to induce the formation of filopodia in HeLa cells. In contrast to wild-type LPR1, these mutant LPR1 proteins are exclusively localized to intracellular membranes. LPR1 C-term DELTA43 mutant, is incapable of generating new filopodia, but rather localizes to pre-existing endogenous protrusions extended by these cells. Subcellular distribution of mutant LPR1 C-term DELTA43 between the plasma membrane and intracellular membranes is highly comparable with that of wild-type LPR1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
RNAi2 reduces expression levels of LPR1 by ca. 60% in SK-OV-3 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane LPR1 is predominantly distributed uniformly along shafts of protrusions, with an occasional enrichment at their tips. LPR1 may be an integral membrane protein link between the actin core and the surrounding lipid layer of a nascent filopodium. LPP3 does not show these types of membrane protrusions Homo sapiens 5886
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
LPR1, Western blot analysis Homo sapiens
38000
-
LPR1, Western blot analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TBJ4 LPR1
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Source Tissue

Source Tissue Comment Organism Textmining
filopodium
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Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ceramide 1-phosphate + H2O LPP1 but not LPR1 Homo sapiens ceramide + phosphate
-
?
lyso-phosphatidic acid + H2O LPP1 but not LPR1 Homo sapiens monoacylglycerol + phosphate
-
?
additional information LPR1 does not hydrolyze phospholipid substrates under conditions that readily support LPP1 activity Homo sapiens ?
-
?
phosphatidic acid + H2O LPP1 but not LPR1 Homo sapiens diacylglycerol + phosphate
-
?
sphingosine 1-phosphate + H2O LPP1 but not LPR1 Homo sapiens sphingosine + phosphate
-
?

Synonyms

Synonyms Comment Organism
lipidphosphatase-related protein 1
-
Homo sapiens
LPP1
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Homo sapiens
LPP2
-
Homo sapiens
LPP3
-
Homo sapiens
LPR1
-
Homo sapiens
PRG3
-
Homo sapiens