Cloned (Comment) | Organism |
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subcloning and expression of wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D398E | site-directed mutagenesis, the mutant is almost inactive, the activity is independent of the substrate concentration, the mutant shows aberrant regulation of lipid composition | Saccharomyces cerevisiae |
D400E | site-directed mutagenesis, the mutant is almost inactive, the activity is independent of the substrate concentration, the mutant shows aberrant regulation of lipid composition | Saccharomyces cerevisiae |
G80R | site-directed mutagenesis, the mutant is almost inactive, the activity is independent of the substrate concentration, the mutant shows aberrant regulation of lipid composition | Saccharomyces cerevisiae |
additional information | a pah1DELTA deletion mutant shows a phenotype of temperature sensitivity, respiratory deficiency, nuclear/endoplasmic reticulum membrane expansion, derepression of INO1 expression, and alterations in lipid composition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic model | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the enzyme plays a major role in the synthesis of triacylglycerols and phospholipids in Saccharomyces cerevisiae, the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology, role of PAH1-encoded PAP1 in lipid synthesis, pathway, overview | ? | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme plays a major role in the synthesis of triacylglycerols and phospholipids in Saccharomyces cerevisiae, the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology, role of PAH1-encoded PAP1 in lipid synthesis, pathway, overview | Saccharomyces cerevisiae | ? | - |
? | |
additional information | PAH1-encoded Mg2+-dependent PAP1 catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol and phosphate, PAP1 contains the catalytic motif DIDGT at residues 398402 and a conserved Gly80 residue | Saccharomyces cerevisiae | ? | - |
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Subunits | Comment | Organism |
---|---|---|
More | PAP1 contains the catalytic motif DIDGT at residues 398402 and a conserved Gly80 residue | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
3-sn-phosphatidate phosphohydrolase | - |
Saccharomyces cerevisiae |
PAP1 | - |
Saccharomyces cerevisiae |