Any feedback?
Literature summary for 3.1.3.4 extracted from
- Phosphatidate phosphatase in rat liver: the relationship between the activities with membrane-bound phosphatidate and aqueous dispersion of phosphatidate (1985), J. Biochem., 97, 45-54.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tween 20 | increases hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, little effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | inhibition of hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | |
| Tween 20 | hydrolysis of phosphatidate bound to microsomal membrane in presence of optimal concentration of 8 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
phosphatidic acid | phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | |
| 0.16 | - |
phosphatidic acid | phosphatidate bound to microsomal membrane | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates hydrolysis of phosphatidate bound to microsomal membrane or phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes, optimal concentration: 8 mM, no effect on hydrolysis of phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidic acid + H2O | phosphatidate bound to microsomal membrane, phosphatidate in sonicated dispersion of organic solvent-disrupted microsomes or phosphatidate dispersed in sonicated microsomal lipid | Rattus norvegicus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
